CD161++CD8+T cells, including the MAIT cell subset, are specifically activated by IL‐12+IL‐18 in a TCR‐independent manner
Open Access
- 10 September 2013
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 44 (1), 195-203
- https://doi.org/10.1002/eji.201343509
Abstract
CD161++CD8+ T cells represent a novel subset that is dominated in adult peripheral blood by mucosal-associated invariant T (MAIT) cells, as defined by the expression of a variable-α chain 7.2 (Vα7.2)-Jα33 TCR, and IL-18Rα. Stimulation with IL-18+IL-12 is known to induce IFN-γ by both NK cells and, to a more limited extent, T cells. Here, we show the CD161++ CD8+ T-cell population is the primary T-cell population triggered by this mechanism. Both CD161++Vα7.2+ and CD161++Vα7.2− T-cell subsets responded to IL-12+IL-18 stimulation, demonstrating this response was not restricted to the MAIT cells, but to the CD161++ phenotype. Bacteria and TLR agonists also indirectly triggered IFN-γ expression via IL-12 and IL-18. These data show that CD161++ T cells are the predominant T-cell population that responds directly to IL-12+IL-18 stimulation. Furthermore, our findings broaden the potential role of MAIT cells beyond bacterial responsiveness to potentially include viral infections and other inflammatory stimuli.Keywords
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