The Bacillus subtilis ydjL ( bdhA ) Gene Encodes Acetoin Reductase/2,3-Butanediol Dehydrogenase
- 15 November 2008
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 74 (22), 6832-6838
- https://doi.org/10.1128/aem.00881-08
Abstract
Bacillus subtilis is capable of producing 2,3-butanediol from acetoin by fermentation, but to date, the gene encoding the enzyme responsible, acetoin reductase/2,3-butanediol dehydrogenase (AR/BDH), has remained unknown. A search of the B. subtilis genome database with the amino acid sequences of functional AR/BDHs from Saccharomyces cerevisiae and Bacillus cereus resulted in the identification of a highly similar protein encoded by the B. subtilis ydjL gene. A knockout strain carrying a ydjL :: cat insertion mutation was constructed, which (i) abolished 2,3-butanediol production in early stationary phase, (ii) produced no detectable AR or BDH activity in vitro, and (iii) accumulated the precursor acetoin in early stationary phase. The ydjL :: cat mutation also affected the kinetics of lactate but not acetate production during stationary-phase cultivation with glucose under oxygen limitation. A very small amount of 2,3-butanediol was detected in very-late-stationary-phase (96-hour) cultures of the ydjL :: cat mutant, suggesting the existence of a second gene encoding a minor AR activity. From the data, it is proposed that the major AR/BDH-encoding gene ydjL be renamed bdhA .Keywords
This publication has 28 references indexed in Scilit:
- Bridges and Chasms: Summary of the IMAGE 2 Meeting in Montreal, Canada, 30 April to 3 May 2007Journal of Bacteriology, 2008
- Isolation and Characterization of Acid-Tolerant, Thermophilic Bacteria for Effective Fermentation of Biomass-Derived Sugars to Lactic AcidApplied and Environmental Microbiology, 2006
- Paenibacillus sp. Strain JDR-2 and XynA 1 : a Novel System for Methylglucuronoxylan UtilizationApplied and Environmental Microbiology, 2006
- Genetic Changes To Optimize Carbon Partitioning between Ethanol and Biosynthesis in EthanologenicEscherichia coliApplied and Environmental Microbiology, 2002
- Characterization of a (2R,3R)-2,3-Butanediol Dehydrogenase as theSaccharomyces cerevisiae YAL060W Gene ProductJournal of Biological Chemistry, 2000
- Adaptation of Bacillus subtilis to oxygen limitationFEMS Microbiology Letters, 1997
- Studies on transformation of Escherichia coli with plasmidsJournal of Molecular Biology, 1983
- Biomass as a Source of Chemical Feedstocks: An Economic EvaluationScience, 1981
- Mutants of Bacillus subtilis Blocked in Acetoin ReductaseJBIC Journal of Biological Inorganic Chemistry, 1973
- A Microbe in International AffairsScientific American, 1927