Measuring the Flight Ability of the Ambrosia Beetle, Platypus Quercivorus (Murayama), Using a Low-Cost, Small, and Easily Constructed Flight Mill
- 6 August 2018
- journal article
- research article
- Published by MyJove Corporation in Journal of Visualized Experiments
- No. 138,p. e57468
- https://doi.org/10.3791/57468
Abstract
The ambrosia beetle, Platypus quercivorus (Murayama), is the vector of a fungal pathogen that causes mass mortality of Fagaceae trees (Japanese oak wilt). Therefore, knowing the dispersal capacity may help inform trapping/tree removal efforts to prevent this disease more effectively. In this study, we measured the flight velocity and duration and estimated the flight distance of the beetle using a newly developed flight mill. The flight mill is low cost, small, and constructed using commonly available items. Both the flight mill arm and its vertical axis comprise a thin needle. A beetle specimen is glued to one tip of the arm using instant glue. The other tip is thick due to being covered with plastic, thus it facilitates the detection of rotations of the arm. The revolution of the arm is detected by a photo sensor mounted on an infrared LED, and is indicated by a change in the output voltage when the arm passed above the LED. The photo sensor is connected to a personal computer and the output voltage data are stored at a sampling rate of 1 kHz. By conducting experiments using this flight mill, we found that P. quercivorus can fly at least 27 km. Because our flight mill comprises cheap and small ordinary items, many flight mills can be prepared and used simultaneously in a small laboratory space. This enables experimenters to obtain a sufficient amount of data within a short period.Keywords
This publication has 9 references indexed in Scilit:
- Phototactic behavior of the ambrosia beetle Platypus quercivorus (Murayama) (Coleoptera: Platypodidae) before and after flightJournal of Insect Behavior, 2017
- A Simple Flight Mill for the Study of Tethered Flight in InsectsJournal of Visualized Experiments, 2015
- Factors Affecting the Flight Capacity of Tetrastichus planipennisi (Hymenoptera: Eulophidae), a Classical Biological Control Agent of Agrilus planipennis (Coleoptera: Buprestidae)Environmental Entomology, 2014
- Patterns of Flight Behavior and Capacity of Unmated Navel Orangeworm (Lepidoptera: Pyralidae) Adults Related to Age, Gender, and Wing SizeEnvironmental Entomology, 2014
- Factors Influencing Flight Capacity of the Mountain Pine Beetle (Coleoptera: Curculionidae: Scolytinae)Environmental Entomology, 2014
- Effect of Binodoxys communis parasitism on flight behavior of the soybean aphid, Aphis glycinesBiological Control, 2012
- Diel Flight Pattern and Flight Performance of Cactoblastis cactorum (Lepidoptera: Pyralidae) Measured on a Flight Mill: Influence of Age, Gender, Mating Status, and Body SizeJournal of Economic Entomology, 2008
- Nutritional value of floral nectar sources for flight in the parasitoid wasp, Cotesia glomerataPhysiological Entomology, 2006
- The effect of age on the flight performance of female Aedes aegypti mosquitoesJournal of Insect Physiology, 1968