Production of dodecanedioic acid via biotransformation of low cost plant-oil derivatives using Candida tropicalis
- 1 October 2017
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Industrial Microbiology & Biotechnology
- Vol. 44 (10), 1491-1502
- https://doi.org/10.1007/s10295-017-1972-6
Abstract
Dodecanedioic acid (DDA) is highly useful to the chemical industry as a versatile precursor for producing the polyamide nylon-6,12, which is used for many technical applications, such as heat and chemical-resistant sheaths. However, DDA synthesis has several drawbacks, such as high energy input and cost-intensive removal of by-products. Therefore, alternative bio-based production routes are required due to increasing industrial demand for green chemicals and renewable products. Candida tropicalis converts petrochemical-based n-dodecanes to the corresponding dicarboxylic acids by targeted functionalization. To increase sustainability of the DDA production process, we tested dodecanoic acid methyl ester, which can be easily obtained from transesterification of coconut oil, in whole-cell biotransformation by C. tropicalis. By modifying selected process parameters, a final DDA concentration of 66 g/L was achieved using a highly reliable, small-scale bioreactor system. Crucial process development included a gradual pH shift, an optimized substrate feeding strategy, and monitoring the transcriptional profile.Keywords
Funding Information
- the Federal Ministry of Education and Research (BMBF) (0316044B)
This publication has 26 references indexed in Scilit:
- High-level productivity of α,ω-dodecanedioic acid with a newly isolated Candida viswanathii strainJournal of Industrial Microbiology & Biotechnology, 2017
- The Properties of Lauric Acid and Their Significance in Coconut OilJournal of Oil & Fat Industries, 2014
- Biotechnological synthesis of long‐chain dicarboxylic acids as building blocks for polymersEuropean Journal of Lipid Science and Technology, 2011
- qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR dataGenome Biology, 2007
- Identification and Characterization of the CYP52 Family of Candida tropicalis ATCC 20336, Important for the Conversion of Fatty Acids and Alkanes to α,ω-Dicarboxylic AcidsApplied and Environmental Microbiology, 2003
- Transformation of Fatty Acids Catalyzed by Cytochrome P450 Monooxygenase Enzymes of Candida tropicalisApplied and Environmental Microbiology, 2003
- In Vitro Killing of Candida albicans by Fatty Acids and MonoglyceridesAntimicrobial Agents and Chemotherapy, 2001
- pH Regulation of Gene Expression in FungiFungal Genetics and Biology, 2000
- Identification and production of 3-hydroxy-Δ 9 - cis -1,18-octadecenedioic acid by mutants of Candida tropicalisApplied Microbiology and Biotechnology, 1996
- Antimicrobial agents derived from fatty acidsJournal of Oil & Fat Industries, 1984