PKC‐β and PKC‐ζ mediate opposing effects on proximal tubule Na+,K+‐ATPase activity
- 28 July 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 456 (1), 45-48
- https://doi.org/10.1016/s0014-5793(99)00925-4
Abstract
Dopamine (DA) inhibits rodent proximal tubule Na+,K+-ATPase via stimulation of protein kinase C (PKC). However, direct stimulation of PKC by phorbol 12-myristate 13-acetate (PMA) results in increased Na+,K+-ATPase. LY333531, a specific inhibitor of the PKC-β isoform, prevents PMA-dependent activation of Na+,K+-ATPase, but has no effect on DA inhibition of this activity. A similar result was obtained with a PKC-β inhibitor peptide. Concentrations of staurosporine, that inhibits PKC-ζ, prevent DA-dependent inhibition of Na+,K+-ATPase and a similar effect was obtained with a PKC-ζ inhibitor peptide. Thus, PMA-dependent stimulation of Na+,K+-ATPase is mediated by activation of PKC-β, whereas inhibition by DA requires activation of PKC-ζ.Keywords
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