Analysis of Serum miRNA Profiles of Myasthenia Gravis Patients
Open Access
- 17 March 2014
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 9 (3), e91927
- https://doi.org/10.1371/journal.pone.0091927
Abstract
Myasthenia gravis (MG) is an autoimmune disease characterized by the presence of autoantibodies, mainly against the acetylcholine receptor (AChR). The mechanisms triggering and maintaining this chronic disease are unknown. MiRNAs are regulatory molecules that play a key role in the immune system and are altered in many autoimmune diseases. The aim of this study was to evaluate miRNA profiles in serum of 61 AChR MG patients. We studied serum from patients with early onset MG (n = 22), late onset MG (n = 27) and thymoma (n = 12), to identify alterations in the specific subgroups. In a discovery cohort, we analysed 381 miRNA arrays from 5 patients from each subgroup, and 5 healthy controls. The 15 patients had not received any treatment. We found 32 miRNAs in different levels in MG and analysed 8 of these in a validation cohort that included 46 of the MG patients. MiR15b, miR122, miR-140-3p, miR185, miR192, miR20b and miR-885-5p were in lower levels in MG patients than in controls. Our study suggests that different clinical phenotypes in MG share common altered mechanisms in circulating miRNAs, with no additional contribution of the thymoma. MG treatment intervention does not modify the profile of these miRNAs. Novel insights into the pathogenesis of MG can be reached by the analysis of circulating miRNAs since some of these miRNAs have also been found low in MG peripheral mononuclear cells, and have targets with important roles in B cell survival and antibody production.This publication has 87 references indexed in Scilit:
- MicroRNAs in age‐related diseasesEMBO Molecular Medicine, 2013
- Circulating microRNAs: Association with disease and potential use as biomarkersCritical Reviews in Oncology/Hematology, 2011
- miRNAs as serum biomarkers for Duchenne muscular dystrophyEMBO Molecular Medicine, 2011
- MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteinsNature, 2011
- MicroRNAs Prevent the Generation of Autoreactive AntibodiesImmunity, 2010
- MicroRNA in autoimmunity and autoimmune diseasesJournal of Autoimmunity, 2009
- Autoimmune myasthenia gravis: emerging clinical and biological heterogeneityThe Lancet Neurology, 2009
- Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseasesCell Research, 2008
- Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cellsNature, 2007
- Myasthenia GravisThe New England Journal of Medicine, 1994