Bone Marrow–Derived Mesenchymal Stem Cells in Repair of the Injured Lung
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- 1 August 2005
- journal article
- Published by American Thoracic Society in American Journal of Respiratory Cell and Molecular Biology
- Vol. 33 (2), 145-152
- https://doi.org/10.1165/rcmb.2004-0330oc
Abstract
We sought to determine whether an intact bone marrow is essential to lung repair following bleomycin-induced lung injury in mice, and the mechanisms of any protective effects conferred by bone marrow-derived mesenchymal stem cell (BMDMSC) transfer. We found that myelosupression increased susceptibility to bleomycin injury and that BMDMSC transfer was protective. Protection was associated with the differentiation of engrafted BMDMSC into specific and distinct lung cell phenotypes, with an increase in circulating levels of G-CSF and GM-CSF (known for their ability to promote the mobilization of endogenous stem cells) and with a decrease in inflammatory cytokines. In vitro, cells from injured, but not from normal, mouse lung produced soluble factors that caused BMDMSC to proliferate and migrate toward the injured lung. We conclude that bone marrow stem cells are important in the repair of bleomycin-injured lung and that transfer of mesenchymal stem cells protects against the injury. BMDMSC localize to the injured lung and assume lung cell phenotypes, but protection from injury and fibrosis also involves suppression of inflammation and triggering production of reparative growth factors.This publication has 28 references indexed in Scilit:
- Lung stem cells: New paradigmsExperimental Hematology, 2004
- Chimerism and cure: hematologic and pathologic correction of murine sickle cell diseaseBlood, 2003
- Mobilized Endothelial Progenitor Cells by Granulocyte-Macrophage Colony-Stimulating Factor Accelerate Reendothelialization and Reduce Vascular Inflammation After Intravascular RadiationCirculation, 2003
- Plasticity of marrow-derived stem cellsBlood, 2003
- Mobilization of hematopoietic stem cells during homeostasis and after cytokine exposureBlood, 2003
- A new model of busulphan-induced chronic bone marrow aplasia in the female BALB/c mouseInternational Journal of Experimental Pathology, 2003
- Differentiation, cell fusion, and nuclear fusion during ex vivo repair of epithelium by human adult stem cells from bone marrow stromaProceedings of the National Academy of Sciences of the United States of America, 2003
- Effect of keratinocyte growth factor on the proliferation, clonogenic capacity and colony size of human epithelial tumour cells in vitroInternational Journal of Radiation Biology, 2003
- ‘Green mice’ as a source of ubiquitous green cellsFEBS Letters, 1997
- Tumor necrosis factor/cachectin plays a key role in bleomycin-induced pneumopathy and fibrosis.The Journal of Experimental Medicine, 1989