VAMP/synaptobrevin isoforms 1 and 2 are widely and differentially expressed in nonneuronal tissues.
Open Access
- 1 January 1996
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 132 (1), 167-179
- https://doi.org/10.1083/jcb.132.1.167
Abstract
VAMP/synaptobrevin is part of the synaptic vesicle docking and fusion complex and plays a central role in neuroexocytosis. Two VAMP (vesicle-associated membrane protein) isoforms are expressed in the nervous system and are differently distributed among the specialized parts of the tissue. Here, VAMP-1 and -2 are shown to be present in all rat tissues tested, including kidney, adrenal gland, liver, pancreas, thyroid, heart, and smooth muscle. The two isoforms are differentially expressed in various tissues and their level may depend on differentiation. VAMP-1 is restricted to exocrine pancreas and to kidney tubular cells, whereas VAMP-2 is the predominant isoform present in Langerhans islets and in glomerular cells. Both isoforms show a patchy vesicular intracellular distribution in confocal microscopy. The present results provide evidence for the importance of neuronal VAMP proteins in the physiology of all cells.Keywords
This publication has 64 references indexed in Scilit:
- A post-docking role for synaptobrevin in synaptic vesicle fusionNeuron, 1994
- Implications of the SNARE hypothesis for intracellular membrane topology and dynamicsCurrent Biology, 1994
- Cysteine String Proteins: a Potential Link Between Synaptic Vesicles and Presynaptic Ca 2+ ChannelsScience, 1994
- Differential expression of transcripts from syb, a Drosophila melanogaster gene encoding VAMP (synaptobrevin) that is abundant in non-neuronal cellsGene, 1993
- Much ado about dockingCurrent Biology, 1993
- SNAP receptors implicated in vesicle targeting and fusionNature, 1993
- SNAPs, a family of NSF attachment proteins involved in intracellular membrane fusion in animals and yeastCell, 1990
- Role of an N-ethylmaleimide-sensitive transport component in promoting fusion of transport vesicles with cisternae of the Golgi stackCell, 1988
- EVIDENCE FOR RECYCLING OF SYNAPTIC VESICLE MEMBRANE DURING TRANSMITTER RELEASE AT THE FROG NEUROMUSCULAR JUNCTIONThe Journal of cell biology, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970