Use of 16S ribosomal RNA gene analyses to characterize the bacterial signature associated with poor oral health in West Virginia
Open Access
- 1 March 2011
- journal article
- Published by Springer Science and Business Media LLC in BMC Oral Health
- Vol. 11 (1), 7
- https://doi.org/10.1186/1472-6831-11-7
Abstract
West Virginia has the worst oral health in the United States, but the reasons for this are unclear. This pilot study explored the etiology of this disparity using culture-independent analyses to identify bacterial species associated with oral disease. Bacteria in subgingival plaque samples from twelve participants in two independent West Virginia dental-related studies were characterized using 16S rRNA gene sequencing and Human Oral Microbe Identification Microarray (HOMIM) analysis. Unifrac analysis was used to characterize phylogenetic differences between bacterial communities obtained from plaque of participants with low or high oral disease, which was further evaluated using clustering and Principal Coordinate Analysis. Statistically different bacterial signatures (P<0.001) were identified in subgingival plaque of individuals with low or high oral disease in West Virginia based on 16S rRNA gene sequencing. Low disease contained a high frequency of Veillonella and Streptococcus, with a moderate number of Capnocytophaga. High disease exhibited substantially increased bacterial diversity and included a large proportion of Clostridiales cluster bacteria (Selenomonas, Eubacterium, Dialister). Phylogenetic trees constructed using 16S rRNA gene sequencing revealed that Clostridiales were repeated colonizers in plaque associated with high oral disease, providing evidence that the oral environment is somehow influencing the bacterial signature linked to disease. Culture-independent analyses identified an atypical bacterial signature associated with high oral disease in West Virginians and provided evidence that the oral environment influenced this signature. Both findings provide insight into the etiology of the oral disparity in West Virginia.Keywords
This publication has 16 references indexed in Scilit:
- ARTICLE: Recruitment of rural and cognitively impaired older adults for dental researchSpecial Care in Dentistry, 2010
- Comparison of the microbiological features of chronic and aggressive periodontitisPeriodontology 2000, 2010
- Bacterial diversity in the oral cavity of 10 healthy individualsThe ISME Journal, 2010
- Comparisons of Subgingival Microbial Profiles of Refractory Periodontitis, Severe Periodontitis, and Periodontal Health Using the Human Oral Microbe Identification MicroarrayThe Journal of Periodontology, 2009
- Study protocol of the Center for Oral Health Research in Appalachia (COHRA) etiology studyBMC Oral Health, 2008
- The breadth of bacterial diversity in the human periodontal pocket and other oral sitesPeriodontology 2000, 2006
- Consensus Report Periodontal Diseases: Pathogenesis and Microbial FactorsAnnals of Periodontology, 1996
- Periodontal Disease and Cardiovascular DiseaseThe Journal of Periodontology, 1996
- Severe Periodontitis and Risk for Poor Glycemic Control in Patients with Non‐Insulin‐Dependent Diabetes MellitusThe Journal of Periodontology, 1996
- Actinobacillus actinomycetemcomitans in human periodontal diseaseJournal of Clinical Periodontology, 1985