PRORP proteins support RNase P activity in both organelles and the nucleus in Arabidopsis
- 14 May 2012
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 26 (10), 1022-1027
- https://doi.org/10.1101/gad.189514.112
Abstract
RNase P is an essential enzyme that cleaves the 59 leader sequence of tRNA precursors. RNase Ps were believed until now to occur universally as ribonucleoproteins in organisms performing RNase P activity. Here we find that protein-only RNase P enzymes called PRORP (for proteinaceous RNase P) support RNase P activity in vivo in both organelles and the nucleus in Arabidopsis. Beyond tRNA, PRORP proteins are involved in the maturation of small nucleolar RNA (snoRNA) and mRNA. Finally, ribonucleoprotein RNase MRP is not involved in tRNA maturation in plants. Altogether, our results indicate that ribonucleoprotein enzymes have been entirely replaced by proteins for RNase P activity in plants.Keywords
This publication has 34 references indexed in Scilit:
- The Human Mitochondrial TranscriptomeCell, 2011
- Structure of a bacterial ribonuclease P holoenzyme in complex with tRNANature, 2010
- tRNA biology charges to the frontGenes & Development, 2010
- Conserved and divergent features of the structure and function of La and La-related proteins (LARPs)Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2010
- Unexpected diversity of RNase P, an ancient tRNA processing enzyme: Challenges and prospectsFEBS Letters, 2009
- Processing of a Dicistronic tRNA-snoRNA Precursor: Combined Analysis in Vitro and in Vivo Reveals Alternate Pathways and Coupling to Assembly of snoRNPPlant Physiology, 2009
- The Many Pathways of RNA DegradationCell, 2009
- RNase P without RNA: Identification and Functional Reconstitution of the Human Mitochondrial tRNA Processing EnzymeCell, 2008
- Genome-wide search for yeast RNase P substrates reveals role in maturation of intron-encoded box C/D small nucleolar RNAsProceedings of the National Academy of Sciences of the United States of America, 2008
- Mapping of mitochondrial mRNA termini in Arabidopsis thaliana : t-elements contribute to 5′ and 3′ end formationNucleic Acids Research, 2007