Objective: To assess the safety of a new platelet count threshold for the definition of maternal thrombocytopenia late in pregnancy. Methods: A platelet count was performed in 6770 pregnant women late in pregnancy and in 6103 of their newborns as well as in a control group of 287 age-matched nonpregnant healthy women. Results: The prevalence of maternal thrombocytopenia (platelet count less than 150 × 109/L) was 11.6%. The mean platelet counts (248 compared with 213 × 109/L) and 2.5th percentile (164 compared with 116 × 109/L) were significantly higher in healthy nonpregnant women than in pregnant women. Among thrombocytopenic pregnant women, 621 (79%) had platelet counts between 116 and 149 × 109/L; none (0%; 95% confidence interval 0, 0.6) had complications related to thrombocytopenia, and none of their newborns had severe thrombocytopenia (platelet count less than 20 × 109/L). Conclusion: In healthy pregnant women, a platelet count over 115 × 109/L late in pregnancy does not require further investigation during pregnancy and may be considered a safe threshold.