Mass cultivation and storage of the rhabditid nematodePhasmarhabditis hermaphrodita, a biocontrol agent for slugs
- 1 January 1993
- journal article
- research article
- Published by Taylor & Francis Ltd in Biocontrol Science and Technology
- Vol. 3 (4), 513-521
- https://doi.org/10.1080/09583159309355307
Abstract
The rhabditid nematode Phasmarhabditis hermaphrodita (a parasite capable of killing pest slugs) was grown in vitro, in association with a mixed bacterial flora on foam chips impregnated with a kidney‐based nutrient medium in aerated bags, to provide sufficient numbers for laboratory and field experiments. The feasibility of producing nematodes in liquid culture was investigated using 250 ml flasks. Baffled flasks containing 25 or 50 ml or liquid were found to be better than baffled flasks containing 100 ml or unbaffled flasks. Inoculum densities ranging from 50 to 330 ml‐1 did not affect final yield. Dauer larvae in aerated water died rapidly at temperatures of 26–35°C. Survival was progressively better at 22°C and 15°C, and best at 5°C or 10°C.Keywords
This publication has 4 references indexed in Scilit:
- The rhabditid nematodePhasmarhabditis hermaphroditaas a potential biological control agent for slugsBiocontrol Science and Technology, 1993
- Terrestrial SlugsPublished by Springer Science and Business Media LLC ,1992
- Generalized Linear ModelsPublished by Springer Science and Business Media LLC ,1989
- Large scale production, storage and transport of the insect‐parasitic nematodes Neoaplectana spp. and Heterorhabditis spp.Annals of Applied Biology, 1984