Characterization of plant miRNAs and small RNAs derived from potato spindle tuber viroid (PSTVd) in infected tomato
- 1 December 2010
- journal article
- Published by Walter de Gruyter GmbH in Biological Chemistry
- Vol. 391 (12), 1379-90
- https://doi.org/10.1515/bc.2010.148
Abstract
To defend against invading pathogens, plants possess RNA silencing mechanisms involving small RNAs (miRNAs, siRNAs). Also viroids – plant infectious, non-coding, unencapsidated RNA – cause the production of viroid-specific small RNAs (vsRNA), but viroids do escape the cytoplasmic silencing mechanism. Viroids with minor sequence variations can produce different symptoms in infected plants, suggesting an involvement of vsRNAs in symptom production. We analyzed by deep sequencing the spectrum of vsRNAs induced by the PSTVd strain AS1, which causes strong symptoms such as dwarfing and necrosis upon infection of tomato plants cv Rutgers. Indeed, vsRNAs found with highest frequency mapped to the pathogenicity-modulating domain of PSTVd, supporting an involvement of vsRNAs in symptom production. Furthermore, in PSTVd AS1-infected plants the accumulation of some endogenous miRNAs, which are involved in leaf development via regulation of transcription factors, is suppressed. The latter finding supports the hypothesis that a miRNA-dependent (mis)regula-tion of transcription factors causes the viroid symptoms.Keywords
This publication has 40 references indexed in Scilit:
- Origin, Biogenesis, and Activity of Plant MicroRNAsCell, 2009
- Origins and Mechanisms of miRNAs and siRNAsCell, 2009
- ViroidsCellular Microbiology, 2008
- Small RNA metabolism in ArabidopsisTrends in Plant Science, 2008
- Substantial biases in ultra-short read data sets from high-throughput DNA sequencingNucleic Acids Research, 2008
- The Mechanism Selecting the Guide Strand from Small RNA Duplexes is Different Among Argonaute ProteinsPlant and Cell Physiology, 2008
- In silico identification of conserved microRNAs in large number of diverse plant speciesBMC Plant Biology, 2008
- Genetic analysis reveals functional redundancy and the major target genes of the Arabidopsis miR159 familyProceedings of the National Academy of Sciences of the United States of America, 2007
- Regulation of LANCEOLATE by miR319 is required for compound-leaf development in tomatoNature Genetics, 2007
- A Structured Viroid RNA Serves as a Substrate for Dicer-Like Cleavage To Produce Biologically Active Small RNAs but Is Resistant to RNA-Induced Silencing Complex-Mediated DegradationJournal of Virology, 2007