Ribosome biogenesis factors bind a nuclear envelope SUN domain protein to cluster yeast telomeres
Open Access
- 5 August 2011
- journal article
- Published by Springer Science and Business Media LLC in The EMBO Journal
- Vol. 30 (18), 3799-3811
- https://doi.org/10.1038/emboj.2011.267
Abstract
Two interacting ribosome biogenesis factors, Ebp2 and Rrs1, associate with Mps3, an essential inner nuclear membrane protein. Both are found in foci along the nuclear periphery, like Mps3, as well as in the nucleolus. Temperature‐sensitive ebp2 and rrs1 mutations that compromise ribosome biogenesis displace the mutant proteins from the nuclear rim and lead to a distorted nuclear shape. Mps3 is known to contribute to the S‐phase anchoring of telomeres through its interaction with the silent information regulator Sir4 and yKu. Intriguingly, we find that both Ebp2 and Rrs1 interact with the C‐terminal domain of Sir4, and that conditional inactivation of either ebp2 or rrs1 interferes with both the clustering and silencing of yeast telomeres, while telomere tethering to the nuclear periphery remains intact. Importantly, expression of an Ebp2–Mps3 fusion protein in the ebp2 mutant suppresses the defect in telomere clustering, but not its defects in growth or ribosome biogenesis. Our results suggest that the ribosome biogenesis factors Ebp2 and Rrs1 cooperate with Mps3 to mediate telomere clustering, but not telomere tethering, by binding Sir4.Keywords
This publication has 57 references indexed in Scilit:
- Yeast telomerase and the SUN domain protein Mps3 anchor telomeres and repress subtelomeric recombinationGenes & Development, 2009
- Critical Role of Nucleostemin in Pre-rRNA ProcessingJournal of Biological Chemistry, 2009
- The functional importance of telomere clustering: Global changes in gene expression result from SIR factor dispersionGenome Research, 2009
- Release of yeast telomeres from the nuclear periphery is triggered by replication and maintained by suppression of Ku-mediated anchoringGenes & Development, 2008
- Transcription Factor Substitution during the Evolution of Fungal Ribosome RegulationMolecular Cell, 2008
- Telomere anchoring at the nuclear periphery requires the budding yeast Sad1-UNC-84 domain protein Mps3The Journal of cell biology, 2007
- Immobility, inheritance and plasticity of shape of the yeast nucleusBMC Cell Biology, 2007
- MPS3 mediates meiotic bouquet formation in Saccharomyces cerevisiaeProceedings of the National Academy of Sciences, 2007
- Targeted Comparative RNA Interference Analysis Reveals Differential Requirement of Genes Essential for Cell ProliferationMolecular Biology of the Cell, 2006
- The Sad1-UNC-84 homology domain in Mps3 interacts with Mps2 to connect the spindle pole body with the nuclear envelopeThe Journal of cell biology, 2006