LeishmaniaInfection Impairs β1-Integrin Function and Chemokine Receptor Expression in Mononuclear Phagocytes

Abstract

Leishmaniaspp. are intracellular parasites that cause lesions in the skin, mucosa, and viscera. We have previously shown thatLeishmaniainfection reduces mononuclear phagocyte adhesion to inflamed connective tissue. In this study, we examined the role of adhesion molecules and chemokines in this process. Infection rate (r= −0.826,P= 0.003) and parasite burden (r= −0.917,P= 0.028) negatively correlated to mouse phagocyte adhesion. The decrease (58.7 to 75.0% inhibition,P= 0.005) in phagocyte adhesion to connective tissue, induced byLeishmania, occurred as early as 2 h after infection and was maintained for at least 24 h. Interestingly, impairment of cell adhesion was sustained by phagocyte infection, since it was not observed following phagocytosis of killed parasites (cell adhesion varied from 15.2% below to 24.0% above control levels,P> 0.05). In addition,Leishmaniainfection diminished cell adhesion to fibronectin (54.1 to 96.2%,P< 0.01), collagen (15.7 to 83.7%,P< 0.05), and laminin (59.1 to 82.2%,P< 0.05). The CD11b^hisubpopulation was highly infected (49.6 to 97.3%). Calcium and Mg²⁺replacement by Mn²⁺, a treatment that is known to induce integrins to a high state of affinity for their receptors, reverted the inhibition in adhesion caused byLeishmania. This reversion was completely blocked by anti-VLA4 antibodies. Furthermore, expression of CCR4 and CCR5, two chemokine receptors implicated in cell adhesion, was found to be downregulated 16 h after infection (2.8 to 4.1 times and 1.9 to 2.8 times, respectively). Together, these results suggest that mechanisms regulating integrin function are implicated in the change of macrophage adhesion in leishmaniasis.