Targeted Genome Editing Across Species Using ZFNs and TALENs
- 15 July 2011
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 333 (6040), 307
- https://doi.org/10.1126/science.1207773
Abstract
Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.Keywords
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