Tissue distribution and proinflammatory cytokine gene expression following acute oral exposure to deoxynivalenol: Comparison of weanling and adult mice
- 31 May 2008
- journal article
- research article
- Published by Elsevier BV in Food and Chemical Toxicology
- Vol. 46 (8), 2826-2831
- https://doi.org/10.1016/j.fct.2008.05.016
Abstract
The frequent presence of deoxynivalenol (DON) in cereal-based foods and the high intake of these foods by children raises particular concerns about the relative susceptibility of this subpopulation to adverse effects evoked by this mycotoxin. We tested the hypothesis that both toxicokinetics and proinflammatory cytokine gene expression following a oral DON exposure at 5 mg/kg bw differ between weanling (3–4 wk) and young adult (8–10 wk) female mice. DON was rapidly taken up with maximum plasma concentrations reaching 1.0 μg/ml in adult mice at 15 min, whereas DON levels were approximately twice as much in weanling mice at these times. DON was rapidly cleared in both weanling and adult mice with concentrations being reduced by 78% and 81% of the peak levels, respectively, after 2 h. DON accumulation and clearance in spleen, liver, lung and kidney followed similar kinetics to that of plasma with tissue burdens also reaching twice that of adult mice. When TNF-α, IL-1β and IL-6 mRNAs in spleens (a primary source of systemic proinflammatory cytokines) were used as biomarkers of the DON’s effects, expression of these mRNAs was two to three times greater in weanling than adult mouse. However, differences in proinflammatory cytokine expression were less robust or not apparent in the liver or lung. Taken together, these data suggest that young mice are modestly more susceptible than adult mice to the adverse effects of DON and that this might result from a greater toxin tissue burden.Keywords
This publication has 40 references indexed in Scilit:
- Immunochemical assessment of deoxynivalenol tissue distribution following oral exposure in the mouseToxicology Letters, 2008
- Urinary Deoxynivalenol Is Correlated with Cereal Intake in Individuals from the United KingdomEnvironmental Health Perspectives, 2008
- Drug metabolism in the paediatric population and in the elderlyDrug Discovery Today, 2007
- Assessment factors—Applications in health risk assessment of chemicalsEnvironmental Research, 2007
- p38 mitogen-activated protein kinase mediates IL-8 induction by the ribotoxin deoxynivalenol in human monocytesToxicology and Applied Pharmacology, 2006
- Ribotoxic Stress Response to the Trichothecene Deoxynivalenol in the Macrophage Involves the Src Family Kinase HckToxicological Sciences, 2005
- Epithelial sodium channel (ENaC) subunit mRNA and protein expression in rats with puromycin aminonucleoside-induced nephrotic syndromeClinical Science, 2003
- Differential Cytokine mRNA Expression in Mice after Oral Exposure to the Trichothecene Vomitoxin (Deoxynivalenol): Dose Response and Time CourseToxicology and Applied Pharmacology, 1997
- Induction of Cytokine mRNAs in Mice After Oral Exposure to the Trichothecene Vomitoxin (Deoxynivalenol): Relationship to Toxin Distribution and Protein Synthesis InhibitionToxicology and Applied Pharmacology, 1995
- Metabolic fate and elimination in milk, urine and bile of deoxynivalenol following administration to lactating sheepJournal of Environmental Science and Health, Part B, 1987