Preparative Chromatography and Purification

Abstract

The chiral stationary phases (CSP's) that have proceeded from the work of Dr. Pirkle of the University of Illinois have characteristics especially useful to preparative liquid chromatography: Packings made with such CSP's can be highly efficient, lose efficiency only gradually when overloaded and regain it immediately when overload is removed, and allow the elution order of enantiomers to be reversed. These have been used at Regis in both preparative and analytical chromatography: in demonstration, to purify an enantiomer to 99.9967% optical purity and to determine that degree of optical purity with adequate precision and sensitivity. Both the relevant theory and the experimental work suggest that preparative trace-first chromatography is eminently suited to purification to high degree, and becoming the better suited as the degree of purity increases.