Rapid Cross-Linking of Elastin-like Polypeptides with (Hydroxymethyl)phosphines in Aqueous Solution
- 6 April 2007
- journal article
- research article
- Published by American Chemical Society (ACS) in Biomacromolecules
- Vol. 8 (5), 1463-1470
- https://doi.org/10.1021/bm061059m
Abstract
In situ gelation of injectable polypeptide-based materials is attractive for minimally invasive in vivo implantation of biomaterials and tissue engineering scaffolds. We demonstrate that chemically cross-linked elastin-like polypeptide (ELP) hydrogels can be rapidly formed in aqueous solution by reacting lysine-containing ELPs with an organophosphorous cross-linker, β-[tris(hydroxymethyl)phosphino]propionic acid (THPP) under physiological conditions. The mechanical properties of the cross-linked ELP hydrogels were largely modulated by the molar concentration of lysine residues in the ELP and the pH at which the cross-linking reaction was carried out. Fibroblasts embedded in ELP hydrogels survived the cross-linking process and were viable after in vitro culture for 3 days. DNA quantification of ELP hydrogels with encapsulated fibroblasts indicated that there was no significant difference in DNA content between day 0 and day 3 when ELP hydrogels were formed with an equimolar ratio of THPP and lysine residues of the ELPs. These results suggest that THPP cross-linking may be a biocompatible strategy for the in situ formation of cross-linked hydrogels.Keywords
This publication has 48 references indexed in Scilit:
- Ultra-High Expression of a Thermally Responsive Recombinant Fusion Protein in E. coliBiotechnology Progress, 2006
- Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineeringNature Biotechnology, 2005
- Protein-Based Thermoplastic ElastomersMacromolecules, 2004
- The molecular basis of the temperature‐ and pH‐induced conformational transitions in elastin‐based peptidesPeptide Science, 2002
- Thermoplastic Elastomer Hydrogels via Self-Assembly of an Elastin-Mimetic Triblock PolypeptideAdvanced Functional Materials, 2002
- Synthesis and Characterization of Elastin-Mimetic Protein Gels Derived from a Well-Defined Polypeptide PrecursorMacromolecules, 2000
- Facts and more facts: Molecular Biology Lab Fax: Recombinant DNA (Vol. I); Gene Analysis (Vol. II), edited by T. A. BrownTrends in Biotechnology, 1999
- In vitro and in vivo characterization of a 99mTc complex with tris(hydroxymethyl)phosphine (THP)Nuclear Medicine and Biology, 1996
- Mechanical behavior of articular cartilage in shear is altered by transection of the anterior cruciate ligamentJournal of Orthopaedic Research, 1995
- 2D NMR assignment of hyperfine-shifted resonances in strongly paramagnetic metalloproteins: resting-state horseradish peroxidaseJournal of the American Chemical Society, 1991