Immunophenotypic Profile of AC133-Positive Cells in Bone Marrow, Mobilized Peripheral Blood and Umbilical Cord Blood
- 1 January 2002
- journal article
- research article
- Published by Informa UK Limited in Leukemia & Lymphoma
- Vol. 43 (4), 869-873
- https://doi.org/10.1080/10428190290017015
Abstract
AC133 is a molecule whose expression in the human hematopoietic system is restricted to a subset of CD34+ progenitor/stem cells with long-term repopulating ability. The antigenic features of these cells, like CD34+ cells, are described heterogeneous. The immunophenotypic profile of AC133+ cells, detected by means of dual-color flow cytometry, in bone marrow (BM), cytokine-mobilized peripheral blood (PB) and umbilical cord blood (UCB) was evaluated. The highest percentage of AC133+ cells was detected in mobilized PB despite not significantly different from that found in BM, but both are higher than that found in UCB. In addition, the highest percentage of CD34, HLA-DR and CD33 co-expressing AC133+ cells was observed in mobilized PB. Furthermore, UCB was found to be enriched in CD7+ and CD19+ cells and BM was found to be enriched in AC133+ cells co-expressing CDw90 and CD71. Our data confirm the immunophenotypic heterogeneity of cells expressing AC133 antigen, a promising new stem cell marker to be increasingly used as additional target for alternative identification and separation of early hematopoietic cells.Keywords
This publication has 8 references indexed in Scilit:
- Flow cytometric and functional characterization of AC133+ cells from human umbilical cord bloodBritish Journal of Haematology, 2000
- Expression of VEGFR-2 and AC133 by circulating human CD34+ cells identifies a population of functional endothelial precursorsBlood, 2000
- Inability of Activated Cord Blood T Lymphocytes to Perform Th1-like and Th2-like Responses: Implications for TransplantationJournal of Hematotherapy & Stem Cell Research, 1999
- CD34+AC133+Cells Isolated from Cord Blood are Highly Enriched in Long‐Term Culture‐Initiating Cells, NOD/SCID‐Repopulating Cells and Dendritic Cell ProgenitorsThe International Journal of Cell Cloning, 1998
- AC133, a Novel Marker for Human Hematopoietic Stem and Progenitor CellsBlood, 1997
- A Novel Five-Transmembrane Hematopoietic Stem Cell Antigen: Isolation, Characterization, and Molecular CloningBlood, 1997
- Prominin, a novel microvilli-specific polytopic membrane protein of the apical surface of epithelial cells, is targeted to plasmalemmal protrusions of non-epithelial cellsProceedings of the National Academy of Sciences, 1997
- Phenotypic analysis of functional T‐lymphocyte subtypes and natural killer cells in human cord blood: relevance to umbilical cord blood transplantationBritish Journal of Haematology, 1995