DNA-guided display of proteins and protein ligands for the interrogation of biology
- 17 May 2011
- journal article
- review article
- Published by Royal Society of Chemistry (RSC) in Chemical Society Reviews
- Vol. 40 (12), 5789-5801
- https://doi.org/10.1039/c1cs15054e
Abstract
The self-assembly of nanosized DNA templates—based on formation of duplex, triplex, quadruplex or even pentaplex structures—provides unique opportunities for the controlled presentation of appended functional units. Recently, researchers have recognized the potential of such DNA scaffolds to address questions in the life sciences. In this critical review the focus is on the exploration of proteins. It is shown how different scaffolds can be used to control localization, structure and bioactivity of proteins and protein ligands. Further examples demonstrate that DNA-based recognition can even be used to trigger the formation of protein targeted molecules. Potential and existing applications in protein detection, drug discovery, structural characterization of protein targets as well as in the design of nucleic acid responsive pharmacophores are discussed (107 references).Keywords
This publication has 100 references indexed in Scilit:
- Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase ExperimentJournal of the American Chemical Society, 2010
- In Vitro Selection of a DNA-Templated Small-Molecule Library Reveals a Class of Macrocyclic Kinase InhibitorsJournal of the American Chemical Society, 2010
- Toward a General Approach for RNA-Templated Hierarchical Assembly of Split-ProteinsJournal of the American Chemical Society, 2010
- Immobilization and One-Dimensional Arrangement of Virus Capsids with Nanoscale Precision Using DNA OrigamiNano Letters, 2010
- Creating Protein Affinity Reagents by Combining Peptide Ligands on Synthetic DNA ScaffoldsJournal of the American Chemical Society, 2009
- A general electrochemical method for label-free screening of protein–small molecule interactionsChemical Communications, 2009
- Self-assembled DNA nanostructures for distance-dependent multivalent ligand–protein bindingNature Nanotechnology, 2008
- Synthetic Ligands Discovered by in Vitro SelectionJournal of the American Chemical Society, 2007
- In vitro analysis of DNA–protein interactions by proximity ligationProceedings of the National Academy of Sciences of the United States of America, 2007
- Protein detection using proximity-dependent DNA ligation assaysNature Biotechnology, 2002