Efficient chemoenzymatic synthesis of biotinylated human serum albumin–sialoglycoside conjugates containing O-acetylated sialic acids

Abstract

Sialyl Tn (STn) and sialyl lactoside derivatives containing O-acetylated sialic acid residues have been chemoenzymatically synthesized using a one-pot three-enzyme system and conjugated to biotinylated human serum albumin (HSA) using an adipic acid para-nitrophenyl ester coupling reagent. This approach provides an efficient and general protocol for preparing carbohydrate–protein conjugates containing base-sensitive groups.