Levels of Soluble CD163 and Severity of Malaria in Children in Ghana
- 1 September 2008
- journal article
- Published by American Society for Microbiology in Clinical and Vaccine Immunology
- Vol. 15 (9), 1456-1460
- https://doi.org/10.1128/cvi.00506-07
Abstract
CD163 is an acute-phase-regulated monocyte/macrophage membrane receptor expressed late in inflammation. It is involved in the haptoglobin-mediated removal of free hemoglobin from plasma, has been identified as a naturally soluble plasma glycoprotein with potential anti-inflammatory properties, and is possibly linked to an individual's haptoglobin phenotype. High levels of soluble CD163 (sCD163) in a malaria episode may therefore downregulate inflammation and curb disease severity. In order to verify this, the relationships between sCD163 levels, malaria severity, and selected inflammatory mediators (tumor necrosis factor alpha [TNF-α], interleukin-6 [IL-6], and IL-10) were assessed by enzyme-linked immunosorbent assay using plasma samples obtained from pediatric malaria patients with uncomplicated malaria (UM [ n = 38]), cerebral malaria (CM [ n = 52]), and severe malarial anemia (SA [ n = 55]) during two consecutive malaria transmission seasons (2002 and 2003). Median sCD163 levels were higher in UM (11.9 μg/ml) patients than in SA (7.7 μg/ml; P = 0.010) and CM (8.0 μg/ml; P = 0.031) patients. Levels of sCD163 were also higher in all patient groups than in a group of 81 age-matched healthy controls. The higher sCD163/TNF-α ratio in UM patients, coupled with the fact that sCD163 levels correlated with TNF-α levels in UM patients but not in CM and SA patients, suggests inflammatory dysregulation in the complicated cases. The study showed that sCD163 levels are elevated during acute malaria. High sCD163 levels in UM patients may be due to the induction of higher-level anti-inflammatory responses, enabling them to avoid disease complications. It is also possible that UM patients simply lost their CD163 receptors from macrophages in inflammatory sites while complicated-malaria patients still had their receptors attached to activated macrophages, reflecting ongoing and higher-level inflammation associated with complicated malaria.Keywords
This publication has 25 references indexed in Scilit:
- CD163 is the macrophage scavenger receptor for native and chemically modified hemoglobins in the absence of haptoglobinBlood, 2006
- Phenotypic and functional profiling of human proinflammatory type-1 and anti-inflammatory type-2 macrophages in response to microbial antigens and IFN-γ- and CD40L-mediated costimulationJournal of Leukocyte Biology, 2005
- Hemoglobin Scavenger Receptor CD163 Mediates Interleukin-10 Release and Heme Oxygenase-1 SynthesisCirculation Research, 2004
- The heme-heme oxygenase system: a molecular switch in wound healingBlood, 2003
- CD163: a signal receptor scavenging haptoglobin–hemoglobin complexes from plasmaThe International Journal of Biochemistry & Cell Biology, 2002
- Heme oxygenase-1 mediates the anti-inflammatory effect of interleukin-10 in miceNature Medicine, 2002
- Soluble CD163: a marker molecule for monocyte/macrophage activity in diseaseScandinavian Journal of Clinical and Laboratory Investigation, 2002
- Soluble CD163 Inhibits Phorbol Ester-Induced Lymphocyte ProliferationBiochemical and Biophysical Research Communications, 2001
- HUMAN MONOCYTES EXPRESS CD163, WHICH IS UPREGULATED BY IL-10 AND IDENTICAL TO p155Cytokine, 2000
- A Monoclonal Antibody to a Novel Differentiation Antigen on Human Macrophages Associated with the Down-Regulatory Phase of the Inflammatory ProcessPathobiology, 1987