Tumor necrosis factor alpha stimulates NMDA receptor activity in mouse cortical neurons resulting in ERK‐dependent death
Open Access
- 9 November 2006
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 100 (5), 1407-1420
- https://doi.org/10.1111/j.1471-4159.2006.04330.x
Abstract
Multiple cytokines are secreted in the brain during pro‐inflammatory conditions and likely affect neuron survival. Previously, we demonstrated that glutamate and tumor necrosis factor alpha (TNFα) kill neurons via activation of the N‐methyl‐d‐aspartate (NMDA) and TNFα receptors, respectively. This report continues characterizing the signaling cross‐talk pathway initiated during this inflammation‐related mechanism of death. Stimulation of mouse cortical neuron cultures with TNFα results in a transient increase in NMDA receptor‐dependent calcium influx that is additive with NMDA stimulation and inhibited by pre‐treatment with the NMDA receptor antagonist, dl‐2‐amino‐5‐phosphonovaleric acid, or the α‐amino‐3‐hydroxy‐5‐methylisoxazole‐4‐propionate/kainate receptor antagonist, 6,7‐dinitroquinoxaline‐2,3‐dione. Pre‐treatment with N‐type calcium channel antagonist, ω‐conotoxin, or the voltage‐gated sodium channel antagonist, tetrodotoxin, also prevents the TNFα‐stimulated calcium influx. Combined TNFα and NMDA stimulation results in a transient increase in activity of extracellular signal‐regulated kinases (ERKs) and c‐Jun N‐terminal kinases (JNKs). Specific inhibition of ERKs but not JNKs is protective against TNFα and NMDA‐dependent death. Death is mediated via the low‐affinity TNFα receptor, TNFRII, as agonist antibodies for TNFRII but not TNFRI stimulate NMDA receptor‐dependent calcium influx and death. These data demonstrate how microglial pro‐inflammatory secretions including TNFα can acutely facilitate glutamate‐dependent neuron death.Keywords
This publication has 86 references indexed in Scilit:
- Semaphorin 3A and neurotrophins: a balance between apoptosis and survival signaling in embryonic DRG neuronsJournal of Neurochemistry, 2005
- Autoantigen specific T cells inhibit glutamate uptake in astrocytes by decreasing expression of astrocytic glutamate transporter GLAST: a mechanism mediated by tumor necrosis factor‐αThe FASEB Journal, 2005
- Tumor necrosis-factor-alpha (TNF-α) induces rapid insertion of Ca2+-permeable α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate (AMPA)/kainate (Ca-A/K) channels in a subset of hippocampal pyramidal neuronsExperimental Neurology, 2005
- β-Amyloid-Stimulated Microglia Induce Neuron Death via Synergistic Stimulation of Tumor Necrosis Factor α and NMDA ReceptorsJournal of Neuroscience, 2005
- Transient Phosphatidylinositol 3-Kinase Inhibition Protects Immature Primary Cortical Neurons from Oxidative Toxicity via Suppression of Extracellular Signal-regulated Kinase ActivationJournal of Biological Chemistry, 2004
- TRUSS, a Novel Tumor Necrosis Factor Receptor 1 Scaffolding Protein That Mediates Activation of the Transcription Factor NF-κBMolecular and Cellular Biology, 2003
- MAPKs: new targets for neurodegenerationEmerging Therapeutic Targets, 2003
- Molecular interpretation of ERK signal duration by immediate early gene productsNature, 2002
- Identification and characterization of receptors for tumor necrosis factor-α in the brainBiochemical and Biophysical Research Communications, 1991
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976