The novel 2016 WHONeisseria gonorrhoeaereference strains for global quality assurance of laboratory investigations: phenotypic, genetic and reference genome characterization
Top Cited Papers
Open Access
- 17 July 2016
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 71 (11), 3096-3108
- https://doi.org/10.1093/jac/dkw288
Abstract
Gonorrhoea and MDR Neisseria gonorrhoeae remain public health concerns globally. Enhanced, quality-assured, gonococcal antimicrobial resistance (AMR) surveillance is essential worldwide. The WHO global Gonococcal Antimicrobial Surveillance Programme (GASP) was relaunched in 2009. We describe the phenotypic, genetic and reference genome characteristics of the 2016 WHO gonococcal reference strains intended for quality assurance in the WHO global GASP, other GASPs, diagnostics and research worldwide. The 2016 WHO reference strains (n = 14) constitute the eight 2008 WHO reference strains and six novel strains. The novel strains represent low-level to high-level cephalosporin resistance, high-level azithromycin resistance and a porA mutant. All strains were comprehensively characterized for antibiogram (n = 23), serovar, prolyliminopeptidase, plasmid types, molecular AMR determinants, N. gonorrhoeae multiantigen sequence typing STs and MLST STs. Complete reference genomes were produced using single-molecule PacBio sequencing. The reference strains represented all available phenotypes, susceptible and resistant, to antimicrobials previously and currently used or considered for future use in gonorrhoea treatment. All corresponding resistance genotypes and molecular epidemiological types were described. Fully characterized, annotated and finished references genomes (n = 14) were presented. The 2016 WHO gonococcal reference strains are intended for internal and external quality assurance and quality control in laboratory investigations, particularly in the WHO global GASP and other GASPs, but also in phenotypic (e.g. culture, species determination) and molecular diagnostics, molecular AMR detection, molecular epidemiology and as fully characterized, annotated and finished reference genomes in WGS analysis, transcriptomics, proteomics and other molecular technologies and data analysis.Funding Information
- Department of Reproductive Health and Research
- WHO Headquarters
- Australian Government Department of Health
- Department of Microbiology, South Eastern Area Laboratory Services, New South Wales, Australia
- Örebro County Council Research Committee and Foundation for Medical Research at Örebro University Hospital, Sweden
- Wellcome Trust Sanger Institute
- Wellcome Trust (098051, K08-AI104767)
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases
This publication has 80 references indexed in Scilit:
- High-Level Cefixime- and Ceftriaxone-Resistant Neisseria gonorrhoeae in France: Novel penA Mosaic Allele in a Successful International Clone Causes Treatment FailureAntimicrobial Agents and Chemotherapy, 2012
- A Novel Mechanism of High-Level, Broad-Spectrum Antibiotic Resistance Caused by a Single Base Pair Change in Neisseria gonorrhoeaemBio, 2011
- Review and International Recommendation of Methods for Typing Neisseria gonorrhoeae Isolates and Their Implications for Improved Knowledge of Gonococcal Epidemiology, Treatment, and BiologyClinical Microbiology Reviews, 2011
- Is Neisseria gonorrhoeae Initiating a Future Era of Untreatable Gonorrhea?: Detailed Characterization of the First Strain with High-Level Resistance to CeftriaxoneAntimicrobial Agents and Chemotherapy, 2011
- Molecular and Structural Analysis of Mosaic Variants of Penicillin-Binding Protein 2 Conferring Decreased Susceptibility to Expanded-Spectrum Cephalosporins in Neisseria gonorrhoeae: Role of Epistatic MutationsBiochemistry, 2010
- Fast and accurate long-read alignment with Burrows–Wheeler transformBioinformatics, 2010
- MtrR Modulates rpoH Expression and Levels of Antimicrobial Resistance in Neisseria gonorrhoeaeJournal of Bacteriology, 2009
- Ceftibuten Resistance and Treatment Failure of Neisseria gonorrhoeae InfectionAntimicrobial Agents and Chemotherapy, 2008
- Molecular characterization of Neisseria gonorrhoeae identifies transmission and resistance of one ciprofloxacin-resistant strainAPMIS, 2007
- Global transmission of prolyliminopeptidase-negative Neisseria gonorrhoeae strains: implications for changes in diagnostic strategiesSexually Transmitted Infections, 2006