Canadian experience with detection of bacterial contamination in apheresis platelets
- 21 February 2007
- journal article
- research article
- Published by Wiley in Transfusion
- Vol. 47 (3), 421-429
- https://doi.org/10.1111/j.1537-2995.2007.01131.x
Abstract
BACKGROUND: In Canada, both blood suppliers, Héma‐Québec (HQ) and Canadian Blood Services (CBS), implemented bacterial testing in apheresis platelets (PLTs) with an automated microbial detection system (BacT/ALERT, bioMérieux). STUDY DESIGN AND METHODS: Validation of the BacT/ALERT Classic and 3D systems involved apheresis PLT spiking with different bacteria at concentrations of 10 and 102 colony‐forming units per mL. As of February 2006, more than 95 percent of apheresis PLTs were screened for bacterial contamination at HQ and CBS. Between 3.5 and 10 mL of PLTs is inoculated into BacT/ALERT aerobic culture bottles followed by incubation for a maximum of 7 days. RESULTS: During the validation studies, all bacteria were detected at all concentrations and volumes tested. Upon implementation of bacterial screening, the percentage of initial positive samples at CBS and HQ was 0.09 and 0.07 percent, respectively. The rate of indeterminate cultures was significantly higher at CBS than at HQ, whereas the rates for true‐positive, false‐positive, and false‐negative results did not differ significantly. Six confirmed‐positive cultures, including three coagulase‐negative staphylococci and three Enterobacteriaceae species, were detected and PLT units contaminated with these bacteria were not transfused. The rate of true‐positive cultures was significantly lower than that reported by other blood operators. Unfortunately, failed detection of two contaminated units resulted in septic transfusion reactions. CONCLUSION: Bacterial screening of apheresis PLTs in Canada was successfully implemented, and transfusion of contaminated units was prevented. Rapid bacterial detection systems that could be used before transfusion, however, may further reduce the risk of transfusion reactions.Keywords
This publication has 26 references indexed in Scilit:
- Pathogen inactivation techniquesBest Practice & Research Clinical Haematology, 2006
- Evaluation of pooled cultures for bacterial detection in whole blood–derived plateletsTransfusion, 2005
- Detection of bacterial contamination of platelet components: six years’ experience with the BacT/ALERT systemTransfusion, 2004
- Improving the bacteriological safety of platelet transfusionsTransfusion Medicine Reviews, 2004
- Evaluation of an automated culture systemfor detecting bacterial contamination of platelets: an analysis with 15 contaminating organismsTransfusion, 2001
- Evaluation of donor skin disinfection methodsTransfusion, 1997
- A prospective study of symptomatic bacteremia following platelet transfusion and of its managementTransfusion, 1994
- A prospective microbiologic surveillance program to detect and prevent the transfusion of bacterially contaminated plateletsTransfusion, 1993
- Strategies for the avoidance of bacterial contamination of blood componentsTransfusion, 1993
- Septic reactions to platelet transfusions. A persistent problemPublished by American Medical Association (AMA) ,1991