Genetic diversity of rhizobia associated with Desmodium species grown in China
Open Access
- 8 December 2006
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 44 (3), 286-292
- https://doi.org/10.1111/j.1472-765x.2006.02071.x
Abstract
Aims: Desmodia are leguminous plants used as important forage and herbal medicine in China. Little information is available about the nodule bacteria of Desmodium species. To understand the genetic diversity of rhizobia associated with Desmodium species grown in China, isolates from temperate and subtropical regions were obtained and analysed. Methods and Results: A total of 39 rhizobial strains isolated from 9 Desmodium species grown in China were characterized by PCR‐based 16S rDNA gene and 16S–23S rDNA intergenic spacer gene restriction fragment length polymorphism (RFLP) and 16S rRNA gene sequencing. The results showed high diversity among rhizobia symbiotic with Desmodium species. Most microsymbionts of Desmodium species belonged to Bradyrhizobium closely related to Bradyrhizobium elkanii, Bradyrhizobium japonicum and Bradyrhizobium yuanmingense. Several small groups or single strain were related to Rhizobium, Sinorhizobium or Mesorhizobium. Conclusions: Desmodium species formed nodules with diverse rhizobia in Chinese soils. Significance and Impact of the Study: These results offered the first systematic information about the microsymbionts of desmodia grown in the temperate and subtropical regions of China.Keywords
This publication has 11 references indexed in Scilit:
- Diverse bacteria isolated from root nodules of Phaseolus vulgaris and species within the genera Campylotropis and Cassia grown in ChinaSystematic and Applied Microbiology, 2005
- Bradyrhizobia from Wild Phaseolus , Desmodium , and Macroptilium Species in Northern MexicoApplied and Environmental Microbiology, 2002
- AFLP fingerprinting as a tool to study the genetic diversity of Rhizobium galegae isolated from Galega orientalis and Galega officinalisJournal of Biotechnology, 2001
- Diversity of rhizobia associated with Amorpha fruticosa isolated from Chinese soils and description of Mesorhizobium amorphae sp. nov.International Journal of Systematic and Evolutionary Microbiology, 1999
- Phaseolus vulgaris is a non-selective host for nodulationFEMS Microbiology Ecology, 1998
- Rhizobium hainanense sp. nov., Isolated from Tropical LegumesInternational Journal of Systematic and Evolutionary Microbiology, 1997
- Phylogenetic and Genetic Relationships of Mesorhizobium tianshanense and Related RhizobiaInternational Journal of Systematic and Evolutionary Microbiology, 1997
- Numerical Taxonomy and DNA Relatedness of Tropical Rhizobia Isolated from Hainan Province, ChinaInternational Journal of Systematic and Evolutionary Microbiology, 1994
- Phylogeny of the Legume Family: An Approach to Understanding the Origins of NodulationAnnual Review of Ecology and Systematics, 1994
- The LeguminosaePublished by Springer Science and Business Media LLC ,1981