Effects of in vitro exposure to hay dust on the gene expression of chemokines and cell-surface receptors in primary bronchial epithelial cell cultures established from horses with chronic recurrent airway obstruction
- 1 March 2009
- journal article
- Published by American Veterinary Medical Association (AVMA) in American Journal of Veterinary Research
- Vol. 70 (3), 365-372
- https://doi.org/10.2460/ajvr.70.3.365
Abstract
Objective—To examine effects of in vitro exposure to solutions of hay dust, lipopolysaccharide (LPS), or β-glucan on chemokine and cell-surface receptor (CSR) gene expression in primary bronchial epithelial cell cultures (BECCs) established from healthy horses and horses with recurrent airway obstruction (RAO). Sample Population—BECCs established from bronchial biopsy specimens of 6 RAO-affected horses and 6 healthy horses. Procedures—5-day-old BECCs were treated with PBS solution, hay dust solutions, LPS, or β-glucan for 6 or 24 hours. Gene expression of interleukin (IL)-8, chemokine (C-X-C motif) ligand 2 (CXCL2), IL-1β, toll-like receptor 2, toll-like receptor 4, IL-1 receptor 1, and glyceraldehyde 3–phosphate dehydrogenase was measured with a kinetic PCR assay. Results—Treatment with PBS solution for 6 or 24 hours was not associated with a significant difference in chemokine or CSR expression between BECCs from either group of horses. In all BECCs, treatment with hay dust or LPS for 6 hours increased ...Keywords
This publication has 34 references indexed in Scilit:
- Partial divergence of cytokine mRNA expression in bronchial tissues compared to bronchoalveolar lavage cells in horses with recurrent airway obstructionVeterinary Immunology and Immunopathology, 2008
- Epithelial expression of mRNA and protein for IL-6, IL-10 and TNF-α in endobronchial biopsies in horses with recurrent airway obstructionBMC Veterinary Research, 2008
- Effects of in vitro exposure to hay dust on expression of interleukin-17, -23, -8, and -1β and chemokine (C-X-C motif) ligand 2 by pulmonary mononuclear cells isolated from horses chronically affected with recurrent airway diseaseAmerican Journal of Veterinary Research, 2007
- Epithelial expression of TLR4 is modulated in COPD and by steroids, salmeterol and cigarette smokeRespiratory Research, 2007
- Bronchial mucosal inflammation and upregulation of CXC chemoattractants and receptors in severe exacerbations of asthmaThorax, 2007
- Elevated amount of Toll-like receptor 4 mRNA in bronchial epithelial cells is associated with airway inflammation in horses with recurrent airway obstructionAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2007
- Toll-like receptors and chronic lung diseaseClinical Science, 2005
- Expression and Release of Interleukin-8 by Human Bronchial Epithelial Cells from Patients with Chronic Obstructive Pulmonary Disease, Smokers, and Never-SmokersRespiration, 2003
- Cultures of equine respiratory epithelial cells and organ explants as tools for the study of equine influenza virus infectionArchiv für die gesamte Virusforschung, 2001
- Early neutrophil but not eosinophil or platelet recruitment to the lungs of allergic horses following antigen exposureClinical and Experimental Allergy, 1993