Effects of the Controlled-Released Basic Fibroblast Growth Factor from Chitosan−Gelatin Microspheres on Human Fibroblasts Cultured on a Chitosan−Gelatin Scaffold
- 22 March 2007
- journal article
- research article
- Published by American Chemical Society (ACS) in Biomacromolecules
- Vol. 8 (5), 1446-1455
- https://doi.org/10.1021/bm061025e
Abstract
To provide for prolonged, site-specific delivery of basic fibroblast growth factor (bFGF) to the grafted skin in a convenient manner, biodegradable chitosan−gelatin microspheres containing bFGF were fabricated and incorporated into a porous chitosan−gelatin scaffold in this study. The microspheres are an integral part of the porous three-dimensional scaffolds, and their incorporation does not significantly affect the scaffold porosity and the pore size. The release kinetics of bFGF showed a fast release (23.7%) at the initial phase in the first 2 days, and the ultimate accumulated release was approximately 71.8% by day 14, indicating an extended time course for complete release. Human fibroblasts seeded on chitosan−gelatin scaffolds with and without bFGF-loaded chitosan−gelatin microspheres (bFGF-MS) were incubated in vitro for 2 weeks and showed that, compared to chitosan−gelatin scaffolds alone, the scaffolds with bFGF-MS significantly augmented the proliferation and glycosaminoglycan (GAG) synthesis of human fibroblasts. Moreover, real-time reversed transcribed polymerase chain reaction (RT−PCR) analysis for fibroblast-related extracellular matrix (ECM) gene markers demonstrated that the transcript level of laminin was markedly upregulated by about 9-fold. These results suggest that chitosan−gelatin scaffolds with bFGF-MS possess a promising potential as a tissue engineering scaffold to improve skin regeneration efficacy and to promote vascularization.This publication has 20 references indexed in Scilit:
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