Augmentation of 11β‐hydroxysteroid dehydrogenase type 1 in LPS‐activated J774.1 macrophages – Role of 11β‐HSD1 in pro‐inflammatory properties in macrophages
- 27 November 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 581 (3), 349-354
- https://doi.org/10.1016/j.febslet.2006.11.032
Abstract
Macrophage infiltration in obese adipose tissue provokes local inflammation and insulin resistance. Evidence has accumulated that activation of 11β-HSD1 in adipocytes is critically involved in dysfunction of adipose tissue. However, the potential role of 11β-HSD1 in macrophages still remains unclear. We here demonstrate that a murine macrophage cell line, J774.1 cells expressed 11β-HSD1 mRNA and reductase activity, both of which were augmented by lipopolysaccharide (LPS)-induced cell activation. Three kinds of pharmacological inhibition of 11β-HSD1 in LPS-treated macrophages significantly suppressed the expression and secretion of interleukin 1β, tumor necrosis factor α or monocyte chemoattractant protein 1, thereby highlighting a novel role of 11β-HSD1 in pro-inflammatory properties of activated macrophagesKeywords
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