T cell repertoire remodeling following post-transplant T cell therapy coincides with clinical response
- 14 October 2019
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 129 (11), 5020-5032
- https://doi.org/10.1172/jci128323
Abstract
BACKGROUND. Impaired T cell immunity in transplant recipients is associated with infection-related morbidity and mortality. We recently reported the successful use of adoptive T cell therapy (ACT) against drug-resistant/recurrent cytomegalovirus in solid-organ transplant recipients. METHODS. In the present study, we used high-throughput T cell receptor V beta sequencing and T cell functional profiling to delineate the impact of ACT on T cell repertoire remodeling in the context of pretherapy immunity and ACT products. RESULTS. These analyses indicated that a clinical response was coincident with significant changes in the T cell receptor V beta landscape after therapy. This restructuring was associated with the emergence of effector memory T cells in responding patients, while nonresponders displayed dramatic pretherapy T cell expansions with minimal change following ACT. Furthermore, immune reconstitution included both adoptively transferred clonotypes and endogenous clonotypes not detected in the ACT products. CONCLUSION. These observations demonstrate that immune control following ACT requires significant repertoire remodeling, which may be impaired in nonresponders because of the preexisting immune environment. Immunological interventions that can modulate this environment may improve clinical outcomes.Keywords
Funding Information
- National Health and Medical Research Council (APP1062074,APP1102948)
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