Techniques for cryopreservation of individual or small numbers of human spermatozoa: a systematic review
Open Access
- 5 November 2008
- journal article
- review article
- Published by Oxford University Press (OUP) in Human Reproduction Update
- Vol. 15 (2), 153-164
- https://doi.org/10.1093/humupd/dmn061
Abstract
Despite interest in cryopreservation of individual or small number of human spermatozoa, to date, little data is available as regards its effectiveness. We systematically reviewed the outcome after cryopreservation of individual or small numbers of human spermatozoa in patients with severe male factor of infertility. We searched the MEDLINE, EMBASE, Cochrane Systematic Reviews, CENTRAL, Web of Science, Scopus databases for relevant studies up to June of 2008. The search used terms referring to cryopreservation of small amount of sperm. Included studies were limited to human studies with no language restrictions. We identified 30 reports including 9 carriers used for cryopreservation of small quantities/numbers of human spermatozoa (7 non-biological and 2 biological carriers). A wide variety of cryopreservation vehicles were reported. The recovery rate of spermatozoa cryopreserved in a known small number varied widely from 59 to 100%. Fertilization rates were in the range of 18–67%. Frozen–thawed spermatozoa, using this method, were subsequently used for intracytoplasmic sperm injection in only five studies, with few pregnancies reported so far. To date, there remains no consensus as to the ideal carrier for cryopreservation of small number of spermatozoa for clinical purposes. Cryopreservation of individual or small numbers of human spermatozoa may replace the need for repeated surgical sperm retrieval. A controlled multicenter trial with sufficient follow-up would provide valid evidence of the potential benefit of this approach.Keywords
This publication has 42 references indexed in Scilit:
- Open epididymal sperm aspiration (OESA): minimally invasive surgical technique for sperm retrievalArchivos Espanoles de Urologia, 2006
- Cryopreservation of spermatozoa in alginic acid capsulesFertility and Sterility, 2006
- Comparison of Efficacy of Two Techniques for Testicular Sperm Retrieval in Nonobstructive Azoospermia: Multifocal Testicular Sperm Extraction Versus Multifocal Testicular Sperm AspirationJournal of Andrology, 2006
- Novel method for the cryopreservation of testicular sperm and ejaculated spermatozoa from patients with severe oligospermia: A pilot studyFertility and Sterility, 2004
- Freezing a few testicular spermatozoa retrieved by TESAMolecular and Cellular Endocrinology, 2000
- Intracytoplasmic sperm injection with cryopreserved testicular spermatozoaMolecular and Cellular Endocrinology, 2000
- QUALITY OF CRYOPRESERVED TESTICULAR SPERM IN PATIENTS WITH OBSTRUCTIVE AND NONOBSTRUCTIVE AZOOSPERMIAJournal of Urology, 1999
- Decrease in testosterone blood concentrations after testicular sperm extraction for intracytoplasmic sperm injection in azoospermic menThe Lancet, 1998
- Pregnancy after fertilisation with human testicular spermatozoaThe Lancet, 1993
- Fertilising ability of testicular spermatozoaThe Lancet, 1993