Asymmetric centrosome inheritance maintains neural progenitors in the neocortex
Open Access
- 15 October 2009
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 461 (7266), 947-955
- https://doi.org/10.1038/nature08435
Abstract
Asymmetric divisions of radial glia progenitors produce self-renewing radial glia and differentiating cells simultaneously in the ventricular zone (VZ) of the developing neocortex. Whereas differentiating cells leave the VZ to constitute the future neocortex, renewing radial glia progenitors stay in the VZ for subsequent divisions. The differential behaviour of progenitors and their differentiating progeny is essential for neocortical development; however, the mechanisms that ensure these behavioural differences are unclear. Here we show that asymmetric centrosome inheritance regulates the differential behaviour of renewing progenitors and their differentiating progeny in the embryonic mouse neocortex. Centrosome duplication in dividing radial glia progenitors generates a pair of centrosomes with differently aged mother centrioles. During peak phases of neurogenesis, the centrosome retaining the old mother centriole stays in the VZ and is preferentially inherited by radial glia progenitors, whereas the centrosome containing the new mother centriole mostly leaves the VZ and is largely associated with differentiating cells. Removal of ninein, a mature centriole-specific protein, disrupts the asymmetric segregation and inheritance of the centrosome and causes premature depletion of progenitors from the VZ. These results indicate that preferential inheritance of the centrosome with the mature older mother centriole is required for maintaining radial glia progenitors in the developing mammalian neocortex.Keywords
This publication has 50 references indexed in Scilit:
- Centriole Age Underlies Asynchronous Primary Cilium Growth in Mammalian CellsCurrent Biology, 2009
- Mammalian Par3 Regulates Progenitor Cell Asymmetric Division via Notch Signaling in the Developing NeocortexNeuron, 2009
- Centrosome misorientation reduces stem cell division during ageingNature, 2008
- Asymmetric mitosis: Unequal segregation of proteins destined for degradationProceedings of the National Academy of Sciences of the United States of America, 2008
- Distinct behaviors of neural stem and progenitor cells underlie cortical neurogenesisJournal of Comparative Neurology, 2008
- Cep120 and TACCs Control Interkinetic Nuclear Migration and the Neural Progenitor PoolNeuron, 2007
- From Stem Cell to Embryo without CentriolesCurrent Biology, 2007
- A centrosomal mechanism involving CDK5RAP2 and CENPJ controls brain sizeNature Genetics, 2005
- Asymmetric inheritance of centrosomally localized mRNAs during embryonic cleavagesNature, 2002
- Cleavage orientation and the asymmetric inheritance of notchl immunoreactivity in mammalian neurogenesisCell, 1995