Foxj1 transcription factors are master regulators of the motile ciliogenic program
- 16 November 2008
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Genetics
- Vol. 40 (12), 1445-1453
- https://doi.org/10.1038/ng.263
Abstract
Motile cilia induce fluid movement through their rhythmic beating activity. In mammals, the transcription factor Foxj1 has been implicated in motile cilia formation. Here we show that a zebrafish Foxj1 homolog, foxj1a, is a target of Hedgehog signaling in the floor plate. Loss of Foxj1a compromises the assembly of motile cilia that decorate floor plate cells. Besides the floor plate, foxj1a is expressed in Kupffer's vesicle and pronephric ducts, where it also promotes ciliary differentiation. We show that Foxj1a activates a constellation of genes essential for motile cilia formation and function, and that its activity is sufficient for ectopic development of cilia that resemble motile cilia. We also document that a paralogous gene, foxj1b, is expressed in the otic vesicle and seems to regulate motile cilia formation in this tissue. Our findings identify a dedicated master regulatory role for Foxj1 in the transcriptional program that controls the production of motile cilia.Keywords
This publication has 43 references indexed in Scilit:
- RhoA-mediated apical actin enrichment is required for ciliogenesis and promoted by Foxj1Journal of Cell Science, 2007
- Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephrosDevelopment, 2007
- Jagged2a-Notch Signaling Mediates Cell Fate Choice in the Zebrafish Pronephric DuctPLoS Genetics, 2007
- Cooperation of sonic hedgehog enhancers in midline expressionDevelopmental Biology, 2007
- Genomewide Expression Profiling in the Zebrafish Embryo Identifies Target Genes Regulated by Hedgehog Signaling During Vertebrate DevelopmentGenetics, 2006
- The ciliary proteome database: an integrated community resource for the genetic and functional dissection of ciliaNature Genetics, 2006
- The mouse ortholog of EFHC1 implicated in juvenile myoclonic epilepsy is an axonemal protein widely conserved among organisms with motile cilia and flagellaFEBS Letters, 2005
- Foxj1 is required for apical localization of ezrin in airway epithelial cellsJournal of Cell Science, 2003
- A molecular pathway leading to endoderm formation in zebrafishCurrent Biology, 1999
- Proteins closely similar to flagellar tektins are detected in cilia but not in cytoplasmic microtubulesCell Motility, 1985