Effect of dietary fat saturation and cholesterol on low density lipoprotein degradation by mononuclear cells of Cebus monkeys.
- 1 November 1989
- journal article
- abstracts
- Published by Ovid Technologies (Wolters Kluwer Health) in Arteriosclerosis: An Official Journal of the American Heart Association, Inc.
- Vol. 9 (6), 919-927
- https://doi.org/10.1161/01.atv.9.6.919
Abstract
The mechanism by which dietary unsaturated fatty acids lower low density lipoprotein (LDL) cholesterol is unknown. Unsaturated fatty acids incorporated into the cell membrane can increase membrane fluidity and, as a result, dramatically alter membrane-dependent cell functions. Therefore, we examined the effect of long-term dietary consumption of corn oil and coconut oil with and without cholesterol in amounts equivalent to those of a typical Western diet on the degradation of human LDL by peripheral blood mononuclear cells in Cebus albifrons monkeys. Cellular LDL degradation was dramatically enhanced in the mononuclear cells isolated from animals fed corn oil in comparison with those from animals fed coconut oil. The addition of cholesterol to the diets resulted in a slight attenuation of LDL degradation in the corn oil group while no effect was noted in the coconut oil group. Crossover LDL binding and degradation experiments with LDL isolated from animals fed corn oil diets and coconut oil diets demonstrated increased binding and degradation of LDL in mononuclear cells from animals fed corn oil diets. Enhanced mononuclear cell LDL degradation was accompanied by increased cellular cis-unsaturated fatty acyl content, increased membrane fluidity, and decreased plasma cholesterol. Increased cellular cis-unsaturated fatty acyl content with its concomitant increase in membrane fluidity mirrored the dietary lipid profile of the host animal. A linear relationship was observed between cellular LDL degradation and both cellular cis-unsaturated fatty acyl content and membrane fluidity. These observations parallel results noted in whole-animal LDL catabolic studies with these same animals described elsewhere. These data suggest a novel mechanism by which dietary unsaturated fatty acids exert their LDL-lowering effect.Keywords
This publication has 36 references indexed in Scilit:
- Stabilization of acetylcholine receptor secondary structure by cholesterol and negatively charged phospholipids in membranesBiochemistry, 1987
- Effect of dietary fat saturation on plasma lipoproteins and high density lipoprotein metabolism of the rhesus monkey.JCI Insight, 1987
- Receptor-Mediated Endocytosis: Concepts Emerging from the LDL Receptor SystemAnnual Review of Cell Biology, 1985
- Internalization-defective LDL receptors produced by genes with nonsense and frameshift mutations that truncate the cytoplasmic domainCell, 1985
- Incorporation of palmitic acid or oleic acid into macrophage membrane lipids exerts differential effects on the function of normal mouse peritoneal macrophagesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1984
- Fluidity parameters of lipid regions determined by fluorescence polarizationBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1978
- Interaction of the Acetylcholine (Nicotinic) Receptor Protein from Torpedo marmorata Electric Organ with Monolayers of Pure LipidsEuropean Journal of Biochemistry, 1978
- Cholesterol biosynthesis in human lymphocytes, monocytes, and granulocytesBiochemical and Biophysical Research Communications, 1977
- Study of abnormal plasma low-density lipoprotein in rhesus monkeys with diet-induced hyperlipidemiaBiochemistry, 1976
- Effects of dietary fats on plasma lipids and lipoproteins: an hypothesis for the lipid-lowering effect of unsaturated fatty acidsJCI Insight, 1969