Induction of Neutralizing Antibodies and Immunity in Vaccinated Guinea Pigs by Cyanogen Bromide-Peptides of VP3 of Foot-and-Mouth Disease Virus

Abstract

The specificity of guinea pig antisera against large cyanogen bromide-cleaved peptides of the virus capsid protein VP₃ of foot-and-mouth disease virus type O₁, strain Kaufbeuren has been characterized by double immunodiffusion, virus neutralization and protection tests. Antibodies to purified 146S particles and the cleavage peptides of VP₃ showed an incomplete cross-section against VP₃ peptide antigen when reacted in immunodiffusion tests, indicating that new antigenic determinants are exhibited by the peptides which are not recognized by the antiserum against the native virus proteins.