Gold Nanorods as Colorful Chromogenic Substrates for Semiquantitative Detection of Nucleic Acids, Proteins, and Small Molecules with the Naked Eye
- 23 February 2016
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 88 (6), 3227-3234
- https://doi.org/10.1021/acs.analchem.5b04621
Abstract
Herein, we report for the first time a colorful chromogenic substrate, which displays vivid color responses in the presence of different concentration of analytes. Our investigation reveals that the selective shortening of gold nanorods (AuNRs) could generate a series of distinct colors that covers nearly the whole visible range from 400 nm to 760 nm. These vivid colors can be easily distinguished by the naked eyes; as a result, the accuracy of visual inspection could be greatly improved. Next, we demonstrate the utility of AuNRs as multicolor chromogenic substrate to develop a number of colorimetric immunoassay methods, e.g. multicolor enzyme-linked immunosorbent assay (ELISA), multicolor competitive ELISA, and multicolor magnetic immunoassay (MIA). These methods allow us to visually quantify the concentration of a broad range of target molecules with the naked eye, and the obtanied results are highly consistent with those state-of-the-art techniques that tested by the sophisticated apparatus. These multicolor portable and cost-effective immunoassay approaches could be potentially useful for a number of applications, for example, in-home personal healthcare, on-site environmental monitoring, and food inspection in the field.Keywords
Funding Information
- National Natural Science Foundation of China (21275031, 21277025, 21375021, 21575027)
- Ministry of Education of the People\'s Republic of China (IRT 15R11)
- Foundation of Fujian Educational Committee (JA12039, JA13024)
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