Assessing the adhesion of putative indigenous probiotic lactobacilli to human colonic epithelial cells
Open Access
- 1 January 2011
- journal article
- research article
- Published by Scientific Scholar in Indian Journal of Medical Research
- Vol. 134 (5), 664-71
- https://doi.org/10.4103/0971-5916.90992
Abstract
Background & objectives: Adherence of bacteria to epithelial cells and mucosal surfaces is a key criterion for selection of probiotic. We assessed the adhesion property of selected indigenous probiotic Lactobacillus strains based on their hydrophobicity and ability to adhere to human epithelial cells. Methods: Five human faecal Lactobacillus isolates, one from buffalo milk and one from cheese were assessed for hydrophobicity following the microbial adhesion to hydrocarbons (MATH) method and colonization potentials based on their adherence to Caco2 and HT-29 colonic adenocarcinomal human intestinal epithelial cell lines. Lactobacillus strains that adhered to Caco2 and HT-29 cell lines were quantified by plating after trypsinization and simultaneously the adhered bacteria were also examined microscopically after staining with Geimsa stain and counted in different fields. Results: Among the tested faecal isolates, L. plantarum Lp91 showed maximum percentage hydrophobicity (35.73±0.40 for n-hexadecane and 34.26±0.63 for toluene) closely followed by L. plantarum Lp9 (35.53±0.29 for n-hexadecane and 33.00±0.57 for toluene). Based on direct adhesion to epithelial cells, L. plantarum Lp91 was the most adhesive strain to HT-29 and Caco2 cell lines with per cent adhesion values of 12.8 ± 1.56 and 10.2 ± 1.09, respectively. L. delbrukeii CH4, was the least adhesive with corresponding figures of 2.5 ± 0.37 and 2.6 ± 0.20 per cent on HT-29 and Caco2 cell lines. Adhesion of the six isolated Lactobacillus strain to HT-29 cell and Caco2 lines as recorded under microscope varied between 131.0 ± 13.9 (Lp75) to 342.7 ± 50.52 (Lp91) and 44.7 ± 9.29 (CH4) to 315.7± 35.4 (Lp91), respectively. Interpretation & conclusions: Two Indigenous probiotic Lactobacillus strains (Lp9, Lp91) demonstrated their ability to adhere to epithelial cell and exhibited strong hydrophobicity under in vitro conditions, and thus could have better prospects to colonize the gut with extended transitKeywords
This publication has 17 references indexed in Scilit:
- An In Vitro Study on Bacterial Growth Interactions and Intestinal Epithelial Cell Adhesion Characteristics of Probiotic CombinationsCurrent Microbiology, 2009
- Microbes in Gastrointestinal Health and DiseaseGastroenterology, 2009
- Genes and Molecules of Lactobacilli Supporting Probiotic ActionMicrobiology and Molecular Biology Reviews, 2008
- Identification of a Surface Protein from Lactobacillus reuteri JCM1081 That Adheres to Porcine Gastric Mucin and Human Enterocyte-Like HT-29 CellsCurrent Microbiology, 2008
- Electrophoretic Mobility Distributions of Single-Strain Microbial PopulationsApplied and Environmental Microbiology, 2001
- Surface of Lactic Acid Bacteria: Relationships between Chemical Composition and Physicochemical PropertiesApplied and Environmental Microbiology, 2000
- Lactobacillus acidophilus LA 1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria.Gut, 1994
- Adhesion of human Lactobacillus acidophilus strain LB to human enterocyte-like Caco-2 cellsJournal of General Microbiology, 1992
- Survival of Lactic Acid Bacteria in the Human Stomach and Adhesion to Intestinal CellsJournal of Dairy Science, 1987
- Adherence of Lactobacillus Species to Human Fetal Intestinal CellsJournal of Dairy Science, 1982