MicroRNA-155 Modulates Treg and Th17 Cells Differentiation and Th17 Cell Function by Targeting SOCS1
Open Access
- 16 October 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (10), e46082
- https://doi.org/10.1371/journal.pone.0046082
Abstract
MicroRNA (miR)-155 is a critical player in both innate and adaptive immune responses. It can influence CD4+ T cell lineage choice. To clarify the role of miR-155 in CD4+ CD25+ regulatory T (Treg)/T helper (Th)17 cell differentiation and function, as well as the mechanism involved, we performed gain-and loss-of-function analysis by transfection pre-miR-155 and anti-miR-155 into purified CD4+ T cells. The results showed that miR-155 positively regulated both Treg and Th17 cell differentiation. It also induced the release of interleukin (IL)-17A by Th17 cells, but not the release of IL-10 and transforming growth factor (TGF)-β1 by Treg cells. Furthermore, we found that miR-155 reacted through regulating Janus kinase/signal transducer and activator of transcription (JAK/STAT) rather than TGF-β/mothers against decapentaplegic homolog (SMAD) signaling pathway in the process of Treg and Th17 cells differentiation. This may because suppressors of cytokine signaling (SOCS)1, the important negative regulator of JAK/STAT signaling pathway, was the direct target of miR-155 in this process, but SMAD2 and SMAD5 were not. Therefore, we demonstrated that miR-155 enhanced Treg and Th17 cells differentiation and IL-17A production by targeting SOCS1.This publication has 46 references indexed in Scilit:
- The kinase inhibitory region of SOCS-1 is sufficient to inhibit T-helper 17 and other immune functions in experimental allergic encephalomyelitisJournal of Neuroimmunology, 2011
- Mechanistic Role of MicroRNA-146a in Endotoxin-Induced Differential Cross-Regulation of TLR SignalingThe Journal of Immunology, 2011
- MicroRNA-155 Targets SMAD2 and Modulates the Response of Macrophages to Transforming Growth Factor-βOnline Journal of Public Health Informatics, 2010
- Inducible microRNA-155 Feedback Promotes Type I IFN Signaling in Antiviral Innate Immunity by Targeting Suppressor of Cytokine Signaling 1The Journal of Immunology, 2010
- MicroRNA-155 Promotes Autoimmune Inflammation by Enhancing Inflammatory T Cell DevelopmentImmunity, 2010
- IL‐6: Regulator of Treg/Th17 balanceEuropean Journal of Immunology, 2010
- Diverse Targets of the Transcription Factor STAT3 Contribute to T Cell Pathogenicity and HomeostasisImmunity, 2010
- The Th17/Treg functional imbalance during atherogenesis in ApoE−/− miceCytokine, 2010
- Suppressor of cytokine signaling 1 gene expression and polymorphisms in systemic lupus erythematosusLupus, 2010
- LPS induces KH‐type splicing regulatory protein‐dependent processing of microRNA‐155 precursors in macrophagesThe FASEB Journal, 2009