A new rice breeding method: CRISPR/Cas9 system editing of the Xa13 promoter to cultivate transgene‐free bacterial blight‐resistant rice

Abstract

Since the first successful completion of genome‐based editing in the model plant Arabidopsis using CRISPR/Cas9 in 2013 (Jiang et al., 2013), a large number of agronomic trait‐related genes have been successfully edited in rice, including leaf color‐related genes OsYSA, CAO1, and OsPDS; rice quality‐related genes OsWaxy and OsBADH2; fertility‐related genes OsPMS3; and plant type‐related genes LZAY1 and OsIPA1 (Liu et al., 2017). However, most of the above reports are of one sgRNA mutation to a specific gene, and most of the mutation sites are selected in the gene coding region. In addition, some genes cannot adopt this strategy for editing CDS regions because of their specific functions. For example, the Xa13 gene is a pluripotent gene for recessive resistance to bacterial blight that regulates rice bacterial blight resistance and participates in anthers development.