Human Cutaneous Leishmania in a Mouse Macrophage Line: Propagation and Isolation of Intracellular Parasites
- 12 September 1980
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 209 (4462), 1240-1242
- https://doi.org/10.1126/science.7403880
Abstract
A mouse macrophage line, J774G8, supports continuous and prolific intracellular growth of Leishmania mexicana amazonensis, the etiological agent of a South American cutaneous leishmaniasis. The intracellular parasites from these infected cultures can be isolated with high recovery rate and purity by simple Percoll gradient centrifugation.Keywords
This publication has 2 references indexed in Scilit:
- Leishmania donovani: Promastigote-macrophage surface interactions in vitroExperimental Parasitology, 1979
- Growth of Leishmania donovani Amastigotes in a Continuous Macrophage‐Like Cell Culture*The Journal of Protozoology, 1979