Molecular epidemiology of vancomycin-resistant Enterococcus faecium strains isolated from haematological malignancy patients in a research hospital in Turkey
- 1 June 2010
- journal article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 59 (6), 660-664
- https://doi.org/10.1099/jmm.0.012625-0
Abstract
Infections and outbreaks of vancomycin-resistant enterococci (VRE) still appear to be rare in Turkey. In the present study, VRE strains isolated during an outbreak in a haematology unit of a training and research hospital in Turkey were typed and their antimicrobial-resistance patterns were characterized by molecular methods. Twelve vancomycin-resistantEnterococcus faeciumstrains isolated from patients with haematological malignancies were investigated by PCR for the presence of genes encoding resistance to vancomycin, tetracycline, chloramphenicol, gentamicin and erythromycin. Their clonal relationship was evaluated by PFGE and multilocus sequence typing. All strains were resistant to vancomycin and erythromycin, and had thevanAandermBgenes, respectively. PFGE was used to determine the presence of two pulsotypes and determine their subtypes. Pulsotype A belonged to sequence type (ST) 17 and pulsotype B belonged to ST 78. All strains with thevanAgene were not the same clone, indicating multiple acquisitions of resistant isolates, even over such a short time period.Keywords
This publication has 24 references indexed in Scilit:
- Molecular Characterization of Enterococcus faecalis N06-0364 with Low-Level Vancomycin Resistance Harboring a Novel d -Ala- d -Ser Gene Cluster, vanLAntimicrobial Agents and Chemotherapy, 2008
- Nosocomial outbreak of vancomycin-resistant Enterococcus faecium in a paediatric unit at a Turkish university hospitalJournal of Antimicrobial Chemotherapy, 2008
- First isolation of vancomycin-resistant enteroccoci and spread of a single clone in a university hospital in northwestern TurkeyEuropean Journal of Clinical Microbiology & Infectious Diseases, 2007
- Identification and molecular characterization of Van A-type vancomycin-resistant Enterococcus faecalis in Northeast of BrazilMemórias do Instituto Oswaldo Cruz, 2006
- Molecular Characterization of Vancomycin-Resistant Enterococci from Clinical and Surveillance SpecimensInfection Control & Hospital Epidemiology, 2006
- Spread of ampicillin/vancomycin-resistant Enterococcus faecium of the epidemic-virulent clonal complex-17 carrying the genes esp and hyl in German hospitalsEuropean Journal of Clinical Microbiology & Infectious Diseases, 2005
- Global Spread of Vancomycin-resistantEnterococcus faeciumfrom Distinct Nosocomial Genetic ComplexEmerging Infectious Diseases, 2005
- Eradication of a Large Outbreak of a Single Strain of vanB Vancomycin-ResistantEnterococcus faeciumat a Major Australian Teaching HospitalInfection Control & Hospital Epidemiology, 2004
- Characterization of Tn1547, a composite transposon flanked by the IS16 and IS256-like elements, that confers vancomycin resistance in Enterococcus faecalis BM4281Gene, 1996
- Plasmid-Mediated Resistance to Vancomycin and Teicoplanin in Enterococcus FaeciumNew England Journal of Medicine, 1988