cdc42 regulates the exit of apical and basolateral proteins from the trans-Golgi network
Open Access
- 1 May 2001
- journal article
- research article
- Published by Springer Science and Business Media LLC in The EMBO Journal
- Vol. 20 (9), 2171-2179
- https://doi.org/10.1093/emboj/20.9.2171
Abstract
It is well established that Rho‐GTPases regulate vesicle fusion and fission events at the plasma membrane through their modulatory role on the cortical actin cytoskeleton. In contrast, their effects on intracellular transport processes and actin pools are less clear. It was recently shown that cdc42 associates with the Golgi apparatus in an ARF‐dependent manner, similarly to coat proteins involved in vesicle formation and to several actin‐binding proteins. We report here that mutants of cdc42 inhibited the exit of basolateral proteins from the trans ‐Golgi network (TGN), while stimulating the exit of an apical marker, in two different transport assays. This regulation may result from modulation of the actin cytoskeleton, as GTPase‐deficient cdc42 depleted a perinuclear actin pool that rapidly exchanges with exogenous fluorescent actin.This publication has 40 references indexed in Scilit:
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