Cryptic species within the wheat curl mite Aceria tosichella (Keifer) (Acari : Eriophyoidea), revealed by mitochondrial, nuclear and morphometric data
Open Access
- 1 January 2012
- journal article
- research article
- Published by CSIRO Publishing in Invertebrate Systematics
- Vol. 26 (4), 417-433
- https://doi.org/10.1071/is11037
Abstract
The wheat curl mite (WCM), Aceria tosichella (Keifer, 1969), is one of the primary pests of wheat and other cereals throughout the world. Traditional taxonomy recognises WCM as a single eriophyoid species; however, a recent study suggested that two genetic lineages of WCM in Australia might represent putative species. Here, we investigate WCM populations from different host plants in Australia, South America and Europe and test the hypothesis that WCM is, in fact, a complex of cryptic species. We used morphological data in combination with nucleotide sequences of the mitochondrial cytochromec oxidase subunitI (COI) and nuclear D2 region of 28S rDNA and internal transcribed spacer region (ITS1, ITS2) sequences. The molecular analyses did not support the monophyly of A. tosichella because the outgroup A. tulipae (Keifer, 1938) is grouped within WCM. The molecular datasets indicated the existence of distinct lineages within WCM, with the distances between lineages corresponding to interspecific divergence. Morphological analyses failed to clearly separate WCM populations and lineages, but completely separated A. tulipae from A. tosichella. The results suggest that what has been recognised historically as a single species is, in fact, a complex of several genetically isolated evolutionary lineages that demonstrate potential as cryptic species. Hence, their discrimination using solely morphological criteria may be misleading. These findings are particularly significant because of the economic importance of WCM as a direct pest and vector of plant viruses. The wheat curl mite (WCM), Aceria tosichella (Keifer, 1969), is one of the primary pests of wheat and other cereals throughout the world. Traditional taxonomy recognises WCM as a single eriophyoid species; however, a recent study suggested that two genetic lineages of WCM in Australia might represent putative species. Here, we investigate WCM populations from different host plants in Australia, South America and Europe and test the hypothesis that WCM is, in fact, a complex of cryptic species. We used morphological data in combination with nucleotide sequences of the mitochondrial cytochromec oxidase subunitI (COI) and nuclear D2 region of 28S rDNA and internal transcribed spacer region (ITS1, ITS2) sequences. The molecular analyses did not support the monophyly of A. tosichella because the outgroup A. tulipae (Keifer, 1938) is grouped within WCM. The molecular datasets indicated the existence of distinct lineages within WCM, with the distances between lineages corresponding to interspecific divergence. Morphological analyses failed to clearly separate WCM populations and lineages, but completely separated A. tulipae from A. tosichella. The results suggest that what has been recognised historically as a single species is, in fact, a complex of several genetically isolated evolutionary lineages that demonstrate potential as cryptic species. Hence, their discrimination using solely morphological criteria may be misleading. These findings are particularly significant because of the economic importance of WCM as a direct pest and vector of plant viruses.Keywords
This publication has 74 references indexed in Scilit:
- MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony MethodsMolecular Biology and Evolution, 2011
- Divergence in behaviour between the European corn borer,Ostrinia nubilalis, and its sibling speciesOstrinia scapulalis: adaptation to human harvesting?Proceedings. Biological sciences, 2010
- Diversity of Phylogenetic Information According to the Locus and the Taxonomic Level: An Example from a Parasitic Mesostigmatid Mite GenusInternational Journal of Molecular Sciences, 2010
- New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0Systematic Biology, 2010
- jModelTest: Phylogenetic Model AveragingMolecular Biology and Evolution, 2008
- Character-based DNA barcoding allows discrimination of genera, species and populations in OdonataProceedings. Biological sciences, 2007
- Cryptic animal species are homogeneously distributed among taxa and biogeographical regionsBMC Evolutionary Biology, 2007
- Widespread Discordance of Gene Trees with Species Tree in Drosophila: Evidence for Incomplete Lineage SortingPLoS Genetics, 2006
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequencesJournal of Molecular Evolution, 1980