Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation
Open Access
- 17 March 2023
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Pharmacology
- Vol. 14, 1138762
- https://doi.org/10.3389/fphar.2023.1138762
Abstract
It has been proven that hydrogen has obvious anti-inflammatory effects in animal experiments and clinical practice. However, the early dynamic process of the inflammatory response caused by lipopolysaccharide (LPS) and the anti-inflammatory effect of hydrogen has not been definitively reported. In this study, inflammation in male C57/BL6J mice or RAW264.7 cells was induced with LPS, for which hydrogen was immediately administered until samples were taken. Pathological changes in lung tissue were assessed using hematoxylin and eosin (HE) staining. Levels of inflammatory factors in serum were determined using liquid protein chip. The mRNA levels of chemotactic factors in lung tissues, leukocytes, and peritoneal macrophages were measured by qRT-PCR. The expression levels of IL-1α and HIF-1α were measured by immunocytochemistry. Among the 23 inflammatory factors screened, LPS-induced upregulation of IL-1α etc. was significantly inhibited by hydrogen within 1 hour. The mRNA expression of MCP-1, MIP-1α, G-CSF, and RANTES was inhibited obviously by hydrogen at 0.5 and 1 h in mouse peritoneal macrophages. In addition, hydrogen significantly blocked LPS or H2O2-induced upregulation of HIF-1α, and IL-1α in 0.5 h in RAW264.7 cells. The results suggested that hydrogen is potentially inhibitive against inflammation by inhibiting HIF-1α and IL-1α release at early occurrence. The target of the inhibitive LPS-induced-inflammatory action of hydrogen is chemokines in macrophages in the peritoneal cavity. This study provides direct experimental evidence for quickly controlling inflammation with the translational application of a hydrogen-assisted protocol.This publication has 46 references indexed in Scilit:
- Exotoxins and endotoxins: Inducers of inflammatory cytokinesToxicon, 2018
- Lipopolysaccharide mediates hepatic stellate cell activation by regulating autophagy and retinoic acid signalingAutophagy, 2017
- Hypoxia/IL‐1α axis promotes gastric cancer progression and drug resistanceJournal of Digestive Diseases, 2017
- HIF-1α Dependent Wound Healing Angiogenesis In Vivo Can Be Controlled by Site-Specific Lentiviral Magnetic Targeting of SHP-2Molecular Therapy, 2017
- Hypoxia andP. gingivalisSynergistically Induce HIF-1 and NF-κB Activation in PDL Cells and Periodontal DiseasesMediators of Inflammation, 2015
- Treating inflammation by blocking interleukin-1 in a broad spectrum of diseasesNature Reviews Drug Discovery, 2012
- Molecular Hydrogen Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Mice Through Reducing Inflammation and ApoptosisShock, 2012
- Hydrogen inhalation ameliorates ventilator-induced lung injuryCritical Care, 2010
- LPS Induces Hypoxia-Inducible Factor 1 Activation in Macrophage-Differentiated Cells in a Reactive Oxygen Species–Dependent MannerAntioxidants and Redox Signaling, 2008
- A network-based analysis of systemic inflammation in humansNature, 2005