Generation of a Pool of Human Platelet Lysate and Efficient Use in Cell Culture
- 11 October 2012
- book chapter
- Published by Springer Science and Business Media LLC in Methods in Molecular Biology
- Vol. 946, 349-362
- https://doi.org/10.1007/978-1-62703-128-8_22
Abstract
Human platelets represent a promising source of bioactive substances as growth factors not just for in vivo wound healing and tissue repair, but also for the expansion of human stem and progenitor cells in vitro. The replacement of fetal bovine serum (FBS) as a standard culture supplement by human platelet-derived growth factors now allows for the GMP-compliant implementation of various cell therapeutics in the growing field of regenerative medicine. For this purpose a protocol for the preparation of human platelet lysate (HPL) by several freeze–thaw cycles has been developed, resulting in platelet fragmentation and the release of stored growth factors. By pooling up to 15 U of HPL derived from individual blood donors, a virtually standardized product is achieved. The depletion of platelet particles and fragments in a final centrifugation step reduces the risk of alloimmunization against platelet antigens and the formation of aggregates in cell culture. The successful application of pooled human platelet lysate (pHPL) as a culture medium supplement for the ex vivo propagation of human mesenchymal stem/progenitor cells (MSPCs) and endothelial colony forming progenitor cells (ECFCs) indicates the feasibility of this animal serum-free source of growth factors. Further studies will evaluate efficacy and safety of pHPL.Keywords
This publication has 14 references indexed in Scilit:
- Platelet-Rich PlasmaThe American Journal of Sports Medicine, 2009
- Human Alternatives to Fetal Bovine Serum for the Expansion of Mesenchymal Stromal Cells from Bone MarrowThe International Journal of Cell Cloning, 2009
- Efficacy and safety of the use of autologous plasma rich in platelets for tissue regeneration: a systematic reviewTransfusion, 2008
- PERSPECTIVE ARTICLE: Growth factors and cytokines in wound healingWound Repair and Regeneration, 2008
- Rapid Large-Scale Expansion of Functional Mesenchymal Stem Cells from Unmanipulated Bone Marrow Without Animal SerumTissue Engineering, Part C: Methods, 2008
- Delivering growth factors for therapeuticsTrends in Pharmacological Sciences, 2008
- Humanized System to Propagate Cord Blood-Derived Multipotent Mesenchymal Stromal Cells for Clinical ApplicationRegenerative Medicine, 2007
- Human platelet lysate can replace fetal bovine serum for clinical‐scale expansion of functional mesenchymal stromal cellsTransfusion, 2007
- Platelet lysates promote mesenchymal stem cell expansion: A safety substitute for animal serum in cell-based therapy applicationsJournal of Cellular Physiology, 2005
- Tissue regeneration and in loco administration of platelet derivatives: clinical outcome, heterogeneous products, and heterogeneity of the effector mechanismsTransfusion, 2005