Optical induction of plasticity at single synapses reveals input-specific accumulation of αCaMKII
- 19 August 2008
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 105 (33), 12039-12044
- https://doi.org/10.1073/pnas.0802940105
Abstract
Long-term potentiation (LTP), a form of synaptic plasticity, is a primary experimental model for understanding learning and memory formation. Here, we use light-activated channelrhodopsin-2 (ChR2) as a tool to study the molecular events that occur in dendritic spines of CA1 pyramidal cells during LTP induction. Two-photon uncaging of MNI-glutamate allowed us to selectively activate excitatory synapses on optically identified spines while ChR2 provided independent control of postsynaptic depolarization by blue light. Pairing of these optical stimuli induced lasting increase of spine volume and triggered translocation of alphaCaMKII to the stimulated spines. No changes in alphaCaMKII concentration or cytoplasmic volume were observed in neighboring spines on the same dendrite, providing evidence that alphaCaMKII accumulation at postsynaptic sites is a synapse-specific memory trace of coincident activity.Keywords
This publication has 36 references indexed in Scilit:
- Locally dynamic synaptic learning rules in pyramidal neuron dendritesNature, 2007
- Synaptic Strength of Individual Spines Correlates with Bound Ca2+–Calmodulin-Dependent Kinase IIJournal of Neuroscience, 2007
- A structural mechanism for maintaining the ‘on‐state’ of the CaMKII memory switch in the post‐synaptic densityJournal of Neurochemistry, 2007
- Reversal of Synaptic Memory by Ca2+/Calmodulin-Dependent Protein Kinase II InhibitorJournal of Neuroscience, 2007
- CaMKII translocation requires local NMDA receptor-mediated Ca2+ signalingThe EMBO Journal, 2006
- Transition from Reversible to Persistent Binding of CaMKII to Postsynaptic Sites and NR2BJournal of Neuroscience, 2006
- Activity-driven postsynaptic translocation of CaMKIITrends in Pharmacological Sciences, 2005
- Channelrhodopsin-2, a directly light-gated cation-selective membrane channelProceedings of the National Academy of Sciences of the United States of America, 2003
- Synaptic Modification by Correlated Activity: Hebb's Postulate RevisitedAnnual Review of Neuroscience, 2001
- Driving AMPA Receptors into Synapses by LTP and CaMKII: Requirement for GluR1 and PDZ Domain InteractionScience, 2000