BRAF and KRAS gene mutations in intraductal papillary mucinous neoplasm/carcinoma (IPMN/IPMC) of the pancreas
- 8 May 2007
- journal article
- Published by Elsevier BV in Cancer Letters
- Vol. 249 (2), 242-248
- https://doi.org/10.1016/j.canlet.2006.09.007
Abstract
The Raf/MEK/ERK (MAPK) signal transduction is an important mediator of a number of cellular fates including growth, proliferation, and survival. The BRAF gene is activated by oncogenic RAS, leading to cooperative effects in cells responding to growth factor signals. Our study was performed to elucidate a possible role of BRAF in the development of IPMN (Intraductal Papillary Mucinous Neoplasm) and IPMC (Intraductal Papillary Mucinous Carcinoma) of the pancreas. Mutations of BRAF and KRAS were evaluated in 36 IPMN/IPMC samples and two mucinous cystadenomas by direct genomic sequencing. Exons 1 for KRAS, and 5, 11, and 15 for BRAF were examined. Totally we identified 17 (47%) KRAS mutations in exon 1, codon 12 and one missense mutation (2.7%) within exon 15 of BRAF. The mutations appear to be somatic since the same alterations were not detected in the corresponding normal tissues. Our data provide evidence that oncogenic properties of BRAF contribute to the tumorigenesis of IPMN/IPMC, but at a lower frequency than KRAS.Keywords
This publication has 47 references indexed in Scilit:
- PIK3CA Mutations in Intraductal Papillary Mucinous Neoplasm/Carcinoma of the PancreasClinical Cancer Research, 2006
- Mutations of BRAF and RAS are rare events in germ cell tumoursInternational Journal of Cancer, 2004
- Intraductal Papillary Mucinous Neoplasms of the PancreasAnnals of Surgery, 2004
- Intraductal Papillary Mucinous Neoplasms of the PancreasAnnals of Surgery, 2004
- BRAF and FBXW7 (CDC4, FBW7, AGO, SEL10) Mutations in Distinct Subsets of Pancreatic CancerThe American Journal of Pathology, 2003
- A Raf-1 Mutant That Dissociates MEK/Extracellular Signal-Regulated Kinase Activation from Malignant Transformation and Differentiation but Not ProliferationMolecular and Cellular Biology, 2003
- Mutations of the BRAF gene in human cancerNature, 2002
- Kirsten ras mutations in patients with colorectal cancer: the ‘RASCAL II’ studyBritish Journal of Cancer, 2001
- What Is the True Rate of K-ras Mutation in Carcinoma of the Pancreas?Pancreas, 1998
- c‐Ki‐rasPoint Mutations in Ductectatic‐type Mucinous Cystic Neoplasms of the PancreasJapanese Journal of Cancer Research, 1991