Minimizing acetate formation in E. coli fermentations
- 1 August 2007
- journal article
- review article
- Published by Oxford University Press (OUP) in Journal of Industrial Microbiology & Biotechnology
- Vol. 34 (11), 689-700
- https://doi.org/10.1007/s10295-007-0244-2
Abstract
Escherichia coli remains the best-established production organism in industrial biotechnology. However, when aerobic fermentation runs at high growth rates, considerable amounts of acetate are accumulated as by-product. This by-product has negative effects on growth and protein production. Over the last 20 years, substantial research efforts have been expended on reducing acetate accumulation during aerobic growth of E. coli on glucose. From the onset it was clear that this quest would not be a simple or uncomplicated one. Simple deletion of the acetate pathway reduced the acetate accumulation, but other by-products were formed. This mini review gives a clear outline of these research efforts and their outcome, including bioprocess level approaches and genetic approaches. Recently, the latter seems to have some promising results.Keywords
This publication has 129 references indexed in Scilit:
- Metabolic characterisation of E. coli citrate synthase and phosphoenolpyruvate carboxylase mutants in aerobic culturesBiotechnology Letters, 2006
- Overflow Metabolism in Escherichia coli during Steady-State Growth: Transcriptional Regulation and Effect of the Redox RatioApplied and Environmental Microbiology, 2006
- Enhanced production of human epidermal growth factor by a recombinant Escherichia coli integrated with in situ exchange of acetic acid by macroporous ion-exchange resinJournal of Bioscience and Bioengineering, 2005
- Physiological response of central metabolism inEscherichia colito deletion of pyruvate oxidase and introduction of heterologous pyruvate carboxylaseBiotechnology & Bioengineering, 2005
- Expression of ptsG Encoding the Major Glucose Transporter Is Regulated by ArcA in Escherichia coliJournal of Biological Chemistry, 2004
- High cell density fed-batch cultivation of Escherichia coli using exponential feeding combined with pH-statBioprocess and Biosystems Engineering, 2004
- Control of metabolic interconversion of isocitrate dehydrogenase between the catalytically active and inactive forms inEscherichia coliFEMS Microbiology Letters, 1998
- Flux Adaptations of Citrate Synthase—deficient Escherichia coliaAnnals of the New York Academy of Sciences, 1994
- High cell density and high-productivity microbial fermentationCurrent Opinion in Biotechnology, 1992
- Short‐chain organic acids at pH 5.0 kill Escherichia coli and Salmonella spp. without causing membrane perturbationJournal of Applied Bacteriology, 1991