Colony Multiplex PCR Assay for Identification and Differentiation of Campylobacter jejuni , C. coli , C. lari , C. upsaliensis , and C. fetus subsp . fetus
Open Access
- 1 December 2002
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 40 (12), 4744-4747
- https://doi.org/10.1128/jcm.40.12.4744-4747.2002
Abstract
A multiplex PCR assay was used to simultaneously detect genes from the five major clinically relevant Campylobacter spp. Those genes selected were hipO and 23S rRNA from Campylobacter jejuni ; glyA from each of C. coli , C. lari , and C. upsaliensis ; and sapB2 from C. fetus subsp. fetus . The assay was evaluated with 137 clinical and environmental isolates and was found to be rapid and easy to perform and had a high sensitivity and specificity for characterizing isolates, even in mixed cultures.Keywords
This publication has 18 references indexed in Scilit:
- Direct detection of thermotolerant campylobacters in chicken products by PCR and in situ hybridizationResearch in Microbiology, 2001
- Identification of thermophilic Campylobacter spp. by phenotypic and molecular methodsJournal of Applied Microbiology, 2001
- Rapid detection of Campylobacter fetus by polymerase chain reaction combined with non-radioactive hybridization using an oligonucleotide covalently bound to microwellsMolecular and Cellular Probes, 2000
- Detection of Thermophilic Campylobacter from Sparrows by Multiplex PCR: The Role of Sparrows as a Source of Contamination of Broilers with Campylobacter.The Journal of Veterinary Medical Science, 2000
- Cloning of asapBhomologue (sapB2) encoding a putative 112-kDaCampylobacter fetusS-layer protein and its use for identification and molecular genotypingFEMS Immunology & Medical Microbiology, 1998
- Rapid PCR with nested primers for direct detection ofCampylobacter jejuniin chicken washesMolecular and Cellular Probes, 1997
- Specific detection of Campylobacter lari by PCRJournal of Microbiological Methods, 1997
- Campylobacter jejuni and the Expanding Spectrum of Related InfectionsClinical Infectious Diseases, 1995
- Species-specific DNA probes for Campylobacter species isolated from pigs with proliferative enteritisVeterinary Microbiology, 1990
- Rapid identification of Campylobacter species using oligonucleotide probes to 16S ribosomal RNAMolecular and Cellular Probes, 1989